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本文介绍一种用于检查肝组织内乙型肝炎表面抗原(HBsAg)定位的三色染色法。染液配制1.Mayer 氏苏木精染液2.三色染液:45ml 光绿(Light green)(0.5克溶于100m195%酒精)45ml 俾士麦棕(Bismark brown)(0.5克溶于100m195%酒精)45ml 伊红(Eosin)(0.5克溶于100m195%酒精)0.2ml 饱和锂溶液0.03 克磷钨酸
This article describes a trichrome staining method for examining the localization of Hepatitis B surface antigen (HBsAg) in liver tissue. Dye preparation 1. Mayer’s hematoxylin stain 2. Trichrome: 45ml Light green (0.5g in 100m195% alcohol) 45ml Bismark brown (0.5g in 100m195 % Alcohol) 45 ml Eosin (0.5 g dissolved in 100 m 195% alcohol) 0.2 ml saturated lithium solution 0.03 g Phosphotungstic acid