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目的:了解目前淄博地区新生儿感染巨细胞病毒(HCMV)的现状,找出最佳检测方法,提高诊断水平,并探讨巨细胞病毒对肝功能的损害。方法:采用抗体捕获酶联免疫吸附试验(ELISA)及荧光定量PCR(qPCR)对2011年12月至2012年5月出生的2596例新生儿分别进行血清HCMV-IgM抗体和尿液HCMV-DNA定量检测,并对符合HCMV感染的阳性标本进行肝功能回顾性分析。结果:用ELISA检测血清CMV-IgM阳性29例(1.117%),用qPCR检测尿液HCMV-DNA阳性39例(1.502%),两种方法阳性符合率71.79%,差异有统计学意义(P<0.01);共有40例阳性患儿回顾性分析肝功能指标血清总胆红素(TBIL)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷氨酰转肽酶(GGT)均高于正常值,差异均有统计学意义(P<0.05)。结论:淄博地区新生儿巨细胞病毒感染率较高,危害性大,对新生儿早期检测和诊断十分重要,定量荧光PCR法检测敏感性要高于ELISA法检测,具有良好的应用价值;检测出的HCMV感染患儿肝脏易受病毒侵害,造成肝功能损害。
Objective: To understand the current status of neonatal infection with cytomegalovirus (HCMV) in Zibo, to find the best detection method, to improve the diagnostic level and to explore the cytomegalovirus damage to liver function. Methods: Serum HCMV-IgM antibody and urine HCMV-DNA were quantified in 2596 neonates born between December 2011 and May 2012 by ELISA and qPCR. Detection, and positive samples of HCMV infection were retrospectively analyzed for liver function. Results: Serum CMV-IgM was detected by ELISA in 29 cases (1.117%). The positive rate of HCMV-DNA in urine by qPCR was 39 cases (1.502%). The positive coincidence rate of the two methods was 71.79% 0.01). A total of 40 positive children were retrospectively analyzed for serum levels of total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and glutamyl transpeptidase (GGT) Values, differences were statistically significant (P <0.05). Conclusion: The infection rate of neonatal cytomegalovirus in Zibo area is high and harmful. It is very important for early detection and diagnosis of newborn infants. The sensitivity of quantitative fluorescent PCR assay is higher than that of ELISA assay, which has good application value. The liver of children with HCMV infection is susceptible to viruses and causes liver damage.