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合成了一个衍生于吡咯并吡咯二酮的用于半胱氨酸(Cys)检测的比率荧光探针,用核磁共振谱和质谱鉴定了其结构。探针与Cys作用后荧光发射最大波长由576 nm蓝移至536 nm,荧光颜色由黄色变为绿色,可裸眼直观识别。并且该探针可以专一性地识别Cys而不受其他氨基酸的影响。当Cys溶液浓度在0~300μmol/L内变化时,该探针荧光变化F536/F576符合线性关系(R=0.997),可以实现其定量检测。
A ratio fluorescent probe derived from diketopyrrolopyrrole for cysteine (Cys) detection was synthesized and its structure was characterized by nuclear magnetic resonance and mass spectrometry. After the probe and the Cys effect, the maximum wavelength of fluorescence emission shifts from 576 nm to 536 nm, the fluorescence color changes from yellow to green, which can be visually recognized by the naked eye. And this probe can specifically recognize Cys without being affected by other amino acids. When the concentration of Cys was varied from 0 to 300 μmol / L, the fluorescence change of F536 / F576 was in accordance with the linear relationship (R = 0.997), which could be detected quantitatively.