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目的 探讨miR-23b对三阴性乳腺癌增殖、迁移的影响及可能的作用机制.方法 实时荧光定量PCR检测miR-23b在三阴性乳腺癌组织的表达情况;构建稳定表达miR-23b的三阴性乳腺癌细胞株MDA-MB-231/miR-23b、BT549/miR-23b,采用细胞增殖实验、划痕实验和Transwell迁移实验检测miR-23b对三阴性乳腺癌细胞增殖、迁移的影响;双荧光素酶报告基因检测miR-23b对叉头框C2(FOXC2)的靶向调控作用;实时荧光定量PCR与Western blot检测miR-23b对FOXC2基因表达水平的影响.结果 miR-23b在乳腺癌组织中下调表达,且在三阴性乳腺癌组织中表达显著低于非三阴性乳腺癌;miR-23b可抑制MDA-MB-231和BT549细胞的增殖和迁移;双荧光素酶实验证实miRNA-23b直接靶向FOXC2 3-UTR,进而抑制FOXC2的mRNA和蛋白的表达.结论 miR-23b靶向调节FOXC2的表达抑制三阴性乳腺癌细胞的增殖与迁移.“,”Objective To investigate the effect and mechanism of miR-23b on the proliferation and migration of triple negative breast cancer (TNBC) cells.Methods Real time polymerase chain reaction (PCR) was used to detect the expression of miR-23b in triple negative breast cancer tissues.MDA-MB-231/miR-23b,BT549/miR-23b cell lines are constructed.Proliferation assay,scaling healing experiment and Transwell migration assay were used to detect the effect of miR-23b on the proliferation and migration of triple negative breast cancer cells.Dual-luciferase reporter gene assay was employed to examine the interactions between miR-23b and forkhead box C2 (FOXC2).Real time PCR and Western blot were performed to detect the effect of miR-23b on the expression of FOXC2.Results The expression level of miR-23b in triple negative breast cancer tissues was significantly less than that in adjacent normal tissues.miR-23b could reduced the proliferation and migration of triple negative breast cancer cells.Dual-luciferase assay confirmed that miR-23b could regulate the expression and activity of FOXC2.The expression of FOXC2 in mRNA and protein level was inhibited by miR-23b.Conclusions miR-23b can inhibit the expression of FOXC2 and affect the proliferation and migration of triple negative breast cancer cells.