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目的:动态监测实验性脑出血条件下部分脑区脑匀浆去甲肾上腺素含量的变化规律并探讨其在继发性脑损害中的作用。方法:Wistar大鼠70只,随机为手术组及假手术对照组,每组各35只,每组按出血前、出血后30min,6,12,24,48及72h分为7组,每小组5只。采用胶原酶和肝素联合注入尾状核的方法建立大鼠脑出血模型,利用高效液相-电化学法测定出血前,出血后各个时相点血肿周边、下丘脑及脑干去甲肾上腺素的含量。结果:脑出血后30min血肿周边、下丘脑及脑干去甲肾上腺素的含量开始同步升高,并于24h达峰值,48h开始回落。结论去甲肾上腺素参与了脑出血脑组织继发性损害的病理生理过程。
Objective: To dynamically monitor the changes of norepinephrine content in brain homogenate under experimental cerebral hemorrhage and to explore its role in secondary brain damage. Methods: Seventy Wistar rats were randomly divided into surgery group and sham operation control group, 35 rats in each group. Each group was divided into 7 groups according to pre-bleeding, 30min, 6,12,24,48 and 72h after hemorrhage. Each group 5 only A rat model of intracerebral hemorrhage was established by injecting collagenase and heparin into caudate nucleus. The hematoma around the hematoma, hypothalamus and brainstem noradrenalin before and after hemorrhage were determined by HPLC - HPLC content. Results: The content of norepinephrine in hypothalamus and brainstem around the hematoma 30min after ICH began to increase synchronously, reached its peak at 24h and began to decline at 48h. Conclusion Norepinephrine participates in the pathophysiological process of secondary brain damage in patients with intracerebral hemorrhage.