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目的建立同时测定银黄胶囊中绿原酸、木犀草苷和黄芩苷含量的方法。方法 HPLC等度洗脱法。C18色谱柱;流动相为甲醇-水-磷酸(53∶47∶0.1);流速1.0 m L/min;检测波长328 nm;柱温30℃。结果绿原酸在2.200~44.00μg/m L范围内(r2=0.999 9)、木犀草苷在0.080~22.00μg/m L范围内(r2=0.999 5)、黄芩苷在24.20~242.0μg/m L范围内(r2=0.999 2)线性关系均良好;其平均回收率分别为99.6%、99.3%和99.7%。结论该方法操作简便,成本低,工作效率高,可作为银黄胶囊质量的控制方法。
Objective To establish a method for simultaneous determination of chlorogenic acid, luteolin and baicalin in Yinhuang Capsules. Methods HPLC isocratic elution method. C18 column. The mobile phase was methanol-water-phosphoric acid (53:47:0.1). The flow rate was 1.0 m L / min. The detection wavelength was 328 nm. The column temperature was 30 ℃. Results The concentration of chlorogenic acid in the range of 2.200 ~ 44.00 μg / m L (r2 = 0.999 9), luteolin in the range of 0.080-22.00 μg / m L (r2 = 0.999 5) and baicalin in the range of 24.20 ~ 242.0 μg / L (r2 = 0.999 2). The average recoveries were 99.6%, 99.3% and 99.7%, respectively. Conclusion The method is simple, low cost, high efficiency and can be used as the quality control method of Yinhuang capsule.