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目的利用基因芯片技术研究辐射相对不敏感细胞人口腔上皮癌KB细胞受电离辐射后基因表达谱变化。方法①克隆形成实验分析不同照射剂量对KB细胞生长抑制率的影响;②采用基因芯片技术筛选与空白对照组差异表达基因并分析。结果在基因芯片上48 000个基因中,共有749个差异表达的基因,其中上调表达269个,下调表达480个,表达差异的基因主要涉及细胞分裂、核酸合成修复、核酸结合转运、凋亡等多个方面。结论辐射对细胞造成的损伤是多靶点多层次的,深入研究这些辐射相关基因的作用,可为进一步研究辐射敏感或抗性产生的原因提供依据。
OBJECTIVE: To study the gene expression profiles of KB cells exposed to ionizing radiation in human oral epithelial carcinoma cells irradiated with relatively insensitive cells using gene chip technique. Methods (1) Clone formation assay was used to analyze the effect of different doses of Radiation on the growth inhibition rate of KB cells; (2) The genes differentially expressed in control group were screened by gene chip technology and analyzed. Results There were 749 differentially expressed genes among the 48 000 genes in the gene chip, including 269 up-regulated genes and 480 down-regulated genes. The differentially expressed genes mainly involved in cell division, nucleic acid synthesis and repair, nucleic acid binding and transport, apoptosis, etc. Many aspects. Conclusion Radiation damage to cells is multi-target and multi-level. Studying the effect of these radiation-related genes in depth can provide the basis for further study on the causes of radiation sensitivity or resistance.