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方法:腰椎穿刺从蛛网膜下腔抽出脑脊液2毫升,滴进1％氯化铜醇溶液10毫升的试管中,边滴边摇,在室温下放置15分钟后,通过细孔或无灰滤纸过滤,将滤液倾入培养皿并置于真空干燥箱(160～170毫米汞柱,36～37℃)12小时,使形成结晶,然后用放大40～60倍的显微镜照相,根据结晶图的形状及结构诊断各种脑和脊髓疾病,并与脑脊液的细胞数及蛋白含量检查作比较。正常脑脊液与1％清洁的氯化铜醇溶液的结晶图一样,即在光亮的背景上出现透明的长圆柱形结晶体,形成许多结晶中心,均匀地分布于培养皿的各部分。神经系统肿瘤患者脑脊液的结晶不那么透明,可看到许多结晶体缠得过紧及被截断,从而出现结晶的分离和融合。中枢神经系统恶性肿瘤时在不透明的晶
METHODS: Lumbar puncture was performed by removing 2 ml of cerebrospinal fluid from the subarachnoid space and dropping it into a 10-ml tube of a 1% cupric chloride alcohol solution. After shaking for 15 minutes at room temperature, it was filtered through a fine pore or an ashless filter paper The filtrate was poured into petri dishes and placed in a vacuum oven (160-170 mm Hg, 36-37 ° C) for 12 hours to crystallize and then photographed with a magnification of 40 to 60 times. According to the shape and Structural diagnosis of various brain and spinal cord diseases, and cerebrospinal fluid cell number and protein content for comparison. The normal cerebrospinal fluid is the same as the 1% clean copper chloride alcohol solution in the form of a crystallized, transparent, long cylindrical crystal appearing on a bright background, forming many crystalline centers evenly distributed throughout the dish. Cerebrospinal fluid in patients with neurological tumors is not so transparent, and many crystals can be seen tangled and severed, resulting in the separation and fusion of crystals. Central nervous system malignant tumors in opaque crystals