Combination of phase information from isomorphous crystal of R-phycoerythrin from Polysiphonia Urceo

• 作 者: 万柱礼 常文瑞 江涛 宋海卫 张季平 汪曙光 梁栋材
• 机 构: Institute of Biophysics, Chinese Academy of Sciences, National Laboratory of Biomacromolecules, Beij
• 出 处: Progress in Natural Science
• 发表日期: 1996年04期
• 关键词: hydroxide determining figure enough refined finding Patterson diffractometer coo

On the basis of two isoraorphous heavy atom derivatives of R-phycoerythrin (R-PE), both new derivatives of R-PE have also been obtained m 0.05mol/L sodium phosphate buffer and (NH4)2SO4 system containing k2PtCl4 (PTC4) and phenyl mercuric hydroxide (PHMH) reagents by soaking native crystal method. Four heavy atom derivatives were used for phase determination. The overall figure of merit for MIR phases increased to = 0.86 after the solvent flattening. The electron density map calculated is good enough for finding and determining the boundaries of whole molecule and subunits. On the basis of two isoramorph heavy atom derivatives of R-phycoerythrin (R-PE), both new derivatives of R-PE have also been obtained m 0.05 mol / L sodium phosphate buffer and (NH4) 2SO4 system containing k2PtCl4 (PTC4) and The overall figure of merit for MIR phases increased to = 0.86 after the solvent flattening. The electron density map was calculated as good enough for finding and determining the boundaries of whole molecule and subunits