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目的:观察原发性肝内胆管结石患者肝细胞内匍萄糖醛酸转移酶(UDP-GT)活性变化和该酶mRNA表达.方法:①用UDP-葡萄糖醛酸和2-氨基酚为底物,测定肝组织均浆UDP-GT活性.②地高辛标记cDNA探针与肝组织石蜡切片进行原位杂交,图像分析仪进行灰度测定,观察mRNA的表达.结果:患者组27%的患者UDP-GT活性降低,而正常对照组仅有14%的人降低;UDP-GT mRNA的表达虽有降低趋势,但统计学分析差异不显著.结论:原发性肝内胆管结石部分患者肝细胞内UDP-GT活性降低.
OBJECTIVE: To observe the change of intracellular UDP-GT activity and mRNA expression in hepatic cells of patients with primary intrahepatic bile duct stones.Methods: ①Using UDP-glucuronic acid and 2-aminophenol as the base The activity of UDP-GT in the homogenate of the liver tissue was determined by enzyme-linked immunosorbent assay (ELISA) .②The digoxigenin-labeled cDNA probe and the paraffin sections of the liver tissue were in situ hybridized and the gray scale was measured by an image analyzer to observe the mRNA expression.Results: The activity of UDP-GT decreased in patients with normal intrahepatic biliary cirrhosis, while only 14% in normal control group decreased, while the expression of UDP-GT mRNA decreased, but the difference was not statistically significant.Conclusion: Intracellular UDP-GT activity decreased.