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目的研究七叶皂苷钠对氧化低密度脂蛋白(ox-LDL)诱导的人单核细胞系U937细胞所产生的一氧化氮(NO)以及对肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)表达的影响。方法将培养的U937细胞分为正常细胞对照组、ox-LDL组和七叶皂苷钠(20、10、5μg.mL-1)药物干预组。ox-LDL组是将U937细胞与40mg.L-1的ox-LDL共同孵育24h;药物干预组是将不同质量浓度的七叶皂苷钠与40mg.L-1的ox-LDL同时加入培养的U937细胞,共同孵育24h。应用硝酸还原酶法测定NO的分泌量,酶联免疫吸附法测定TNF-α和IL-6的蛋白表达。结果 ox-LDL可刺激U937细胞分泌NO,并促使TNF-α和IL-6的表达增加;20、10μg.mL-1的七叶皂苷钠能够降低ox-LDL诱导的上述炎症因子的升高。结论七叶皂苷钠能抑制ox-LDL诱导的U937细胞表达NO、TNF-α和IL-6。
Aim To investigate the effects of sodium aescinate on nitric oxide (NO) production by ox-LDL-induced human monocytic U937 cells and the effects on tumor necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) expression. Methods U937 cells were divided into normal control group, ox-LDL group and sodium aescinate (20, 10, 5μg.mL-1) drug intervention group. The ox-LDL group was incubated with 40 mg.L-1 ox-LDL for 24 h. The drug intervention group was treated with different concentrations of sodium aescinate and 40 mg.L-1 of ox-LDL simultaneously in U937 Cells were incubated together 24h. Nitric acid reductase method was used to determine the amount of NO secretion, and the protein expression of TNF-α and IL-6 was measured by enzyme-linked immunosorbent assay. Results Ox-LDL stimulated NO secretion in U937 cells and increased the expression of TNF-α and IL-6. Sodium aescinate at 20 and 10 μg.mL-1 reduced the above-mentioned inflammatory factors induced by ox-LDL. Conclusion Sodium aescinate can inhibit the expression of NO, TNF-α and IL-6 in U937 cells induced by ox-LDL.