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本文采用ＥＬＩＳＡ方法，研究了重组的人粒细胞一巨噬细胞集落刺激因子（ｒｈＧＭ－ＣＳＦ）对体外培养的正常人外周血单核细胞ＨＬＡ－ＤＲ抗原表达的影响。结果表明ＧＭ－ＣＳＦ能提高单核细胞ＨＬＡ－ＤＲ抗原表达，并且呈剂量依赖关系，最适剂量（５００ｕ／ｍｌ）时能使ＤＲ抗原表达量提高到对照组的２．５倍。ＧＭ－ＣＳＦ对ＨＬＡ－ＤＲ抗原表达的诱导作用并不是通过诱生的ＩＦＮ－γ的作用而实现的，因为有中和活性的ＩＦＮ－γ单抗并不能阻断这种效应。ＧＭ－ＣＳＦ能够增强低剂量ＩＦＮ－γ（５ｕ／ｍｌ）对单核细胞ＨＬＡ－ＤＲ抗原的诱导作用，但未观察到对高剂量ＩＦＮ－γ（１００ｕ／ｍｌ）的ＨＬＡ－ＤＲ抗原的诱导增强作用。
In this study, the effect of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on HLA-DR antigen expression in normal human peripheral blood mononuclear cells cultured in vitro was studied by ELISA. The results showed that GM-CSF could increase the expression of HLA-DR antigen in monocytes in a dose-dependent manner. The optimal dose of 500u / ml could increase the expression of DR antigen to 2.5 times of the control. The induction of HLA-DR antigen expression by GM-CSF is not achieved by the induction of IFN-γ, as IFN-γ mAb with neutralizing activity does not block this effect. GM-CSF enhanced the induction of monocyte HLA-DR antigens by low doses of IFN-γ (5u / ml), but no induction of HLA-DR antigen induction at high doses of IFN-γ (100u / ml) effect.