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目的：探讨标本久置对ELISA法检测乙型肝炎表面抗原的影响。方法筛选强阳性、弱阳性及阴性标本若干份，用ELISA法对同一标本进行当天和隔日检测。结果强阳性及阴性标本放置前后，乙型肝炎表面抗原检测结果相同，无明显差异，而弱阳性标本放置后，出现假阴性。结论标本久置会使乙型肝炎表面抗原的免疫活性减弱，从而遗漏乙型肝炎表面抗原弱阳性者。“,”Objective To explore the specimens for place of ELISA method to detect the ef ect of hepatitis b surface antigen. Methods Screening strong positive, weakly positive and negative number of samples, using the ELISA method for the same specimen test on the same day and day. Results Before and after strong positive and negative samples placed, hepatitis b surface antigen test results the same, no obvious dif erence, and weakly positive specimens after placing, false negative. Conclusion Specimen long rear can make the immune activity of hepatitis b surface antigen is abate, thereby missing the hepatitis b surface antigen weak positive people.