甘薯病毒病调查研究

• 作 者: 杨永嘉 邢继英 张朝伦
• 机 构: 江苏徐淮地区徐州农科所,江苏徐淮地区徐州农科所,江苏徐淮地区徐州农科所
• 出 处: 江苏农业科学
• 发表日期: 1990年02期
• 关键词: 甘薯 病毒 抗血清 酶联免疫试验 斑点 叶型 阳性反应 皱缩 传染率 羽状

1987～1989年共调查了1000多个甘薯品种(系),甘薯病毒病表现症状类型大致有四种:叶片斑点型、卷叶型、叶片皱缩型、叶片黄化型。用嫁接法对各种症状样本进行传染性测定,斑点型症状的传染率为89.5％,卷叶型的为85.7％,皱缩型的为94.1％,黄化型的为88.2％。用斑点酶联免疫试验方法检测28个样本,有18个样本与甘薯羽状斑驳病毒抗血清呈阳性反应,13个与隐潜病毒抗血清呈阳性反应,其中有12个对两种抗血清都呈阳性反应。检测结果明确了羽状斑驳病毒和隐潜病毒在我省甘薯上普遍存在。

From 1987 to 1989, a total of more than 1000 sweetpotato cultivars (lines) were surveyed. There are four types of symptoms of sweet potato virus disease: leaf spot type, leaf roll type, leaf folds type and leaf yellow type. Inoculation assays of various symptoms were carried out by grafting method. The infection rate of spot-like symptoms was 89.5%, curly leaf type was 85.7%, shrinkage type was 94.1% and yellow type was 88.2%. 28 samples were detected by dot ELISA, 18 samples were positive with anther of sweetpotato feather mottle virus, 13 were positive with antisera of latent virus, of which 12 were positive for both antisera Positive reaction. The test results clearly pinnate mottle virus and latent virus in our province sweet potato ubiquitous.