论文部分内容阅读
目的探讨姜黄素诱导雄激素依赖性前列腺癌LNCaP细胞凋亡过程中caspase途径的作用。方法应用CCK8法检测不同浓度(10、20、30、40、50、60μmol/L)姜黄素作用LNCaP细胞12、24、48h后的细胞生长抑制情况。应用Annexin V/PI双染法检测不同浓度(10、20、40μmol/L)姜黄素作用LNCaP细胞24h后的细胞凋亡情况。应用Western blot检测姜黄素作用LNCaP细胞24h后Bax、Bcl-2、Cleaved-caspase-3、Cytochrome C凋亡相关蛋白的变化。结果姜黄素对LNCaP细胞的生长有明显的抑制作用,且呈时间-剂量依赖性;流式细胞术结果显示姜黄素能诱导LNCaP细胞凋亡,10、20、40μmol/L姜黄素作用LNCaP细胞24、48h后细胞凋亡率分别为(6.01±0.95)%、(15.46±1.13)%、(26.13±1.56)%和(11.19±1.74)%、(25.80±2.47)%、(38.72±2.89)%,且呈时间-剂量依赖性(P<0.05);同时姜黄素能够降低LNCaP细胞中Bcl-2的表达,上调Bax、Cytochrome C、Cleavedcaspase-3的表达。结论姜黄素能抑制LNCaP细胞的生长,其作用可能是通过线粒体途径诱导其凋亡。
Objective To investigate the effect of curcumin on caspase pathway in the apoptosis of androgen-dependent prostate cancer LNCaP cells. Methods The cytostatic effect of LNCaP cells treated with curcumin at different concentrations (10, 20, 30, 40, 50, and 60 μmol/L) was detected by CCK8 method at 12, 24, and 48 h. Annexin V/PI double staining method was used to detect the apoptosis of LNCaP cells treated with curcumin at different concentrations (10, 20, 40 μmol/L) for 24 h. The expression of Bax, Bcl-2, Cleaved-caspase-3 and Cytochrome C apoptosis-related proteins in LNCaP cells treated with curcumin was detected by Western blot. Results Curcumin inhibited the growth of LNCaP cells in a dose-and time-dependent manner. Flow cytometry showed that curcumin could induce LNCaP cell apoptosis, and 10, 20, and 40 μmol/L curcumin acted on LNCaP cells 24 After 48h, the cell apoptosis rates were (6.01±0.95)%, (15.46±1.13)%, (26.13±1.56)%, and (11.19±1.74)%, (25.80±2.47)%, (38.72±2.89)%, respectively. It was time-and dose-dependent (P<0.05). At the same time, curcumin could decrease the expression of Bcl-2 in LNCaP cells and up-regulate the expression of Bax, Cytochrome C and Cleaved caspase-3. Conclusion Curcumin can inhibit the growth of LNCaP cells, and its effect may be induced by mitochondrial pathway.