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目的:建立超氧化物歧化酶(superoxide dismutase,SOD)模拟物脂质体包封率的测定方法。方法:利用紫外分光光度法测定SOD模拟物的含量,检测波长为371 nm。以薄膜分散法制备SOD模拟物脂质体,采用葡聚糖凝胶-微柱离心-紫外分光光度法测定其包封率。结果:SOD模拟物在60.0～240.0μg.mL-1范围内线性关系良好(r=0.999 3),微柱离心法能有效地将脂质体与游离药物分离,加样回收率测定结果为(99.18±1.05)%,利用该方法测定的SOD模拟物脂质体包封率为(91±1.63)%。结论:紫外分光光度法可以作为SOD模拟物的含量测定方法,微柱离心法操作简单,重现性好,适用于SOD模拟物脂质体包封率的测定。
Objective: To establish a method for determination of entrapment efficiency of superoxide dismutase (SOD) mimetic liposomes. Methods: The contents of SOD mimics were determined by ultraviolet spectrophotometry with a detection wavelength of 371 nm. The SOD mimic liposomes were prepared by thin film dispersion method. The entrapment efficiency of the SOD mimics liposomes was determined by Sephadex micro - column centrifugation - UV spectrophotometry. Results: The linearity of SOD mimics was good in the range of 60.0 ~ 240.0μg.mL-1 (r = 0.999 3). Microcolumn centrifugation could effectively separate the liposomes from the free drugs. The recovery of the samples was ( 99.18 ± 1.05)%. The entrapment efficiency of SOD mimetic liposomes measured by this method was (91 ± 1.63)%. Conclusion: Ultraviolet spectrophotometry can be used as a method for the determination of SOD mimics. Micro column centrifugation has the advantages of simple operation and good reproducibility. It is suitable for the determination of encapsulation efficiency of SOD mimics.