论文部分内容阅读
目的通过建立动物的近视眼模型,观察近视眼发展期和恢复期屈光状态的改变以及基质金属蛋白酶(MMP-2)的含量变化,探讨小鸡近视眼模型巩膜重塑过程中的生物化学变化机制。设计随机对照实验性研究。研究对象32只2天龄SPF美国进口白种莱航鸡。方法将32只鸡随机分为A、B、C、D四组,每组8只,A、B组为实验组,用透明眼罩遮盖小鸡的右眼进行单眼形觉视觉剥夺,左眼为对照；C、D组为正常对照。21天后去遮盖,检测双眼屈光状态和眼轴,处死A组和C组并分离7mm直径的后巩膜组织；继续饲养B组和D组至去遮盖后21天,检测双眼屈光状态和眼轴后处死并分离7mm直径的后巩膜组织。用酶活性分析法测定所有动物巩膜中MMP-2的含量,并将实验组和对照组的数据进行统计学分析。主要指标遮盖后及去遮盖后小鸡的屈光度、眼轴,巩膜中MMP-2的含量。结果遮盖21天A、B组左右眼屈光度比较,差异有统计学意义(P= 0．000),平均相差(-28．38±6．72)D；A组左右眼眼轴相比差异有统计学意义(P=0．000),平均相差(1．47±0．37)mm；遮盖眼后极部巩膜中的MMP-2含量显著性升高,A组遮盖眼与对侧无遮盖眼间比较差异有统计学意义,平均增加(76．00±15．20)％。去遮盖21天B组左右眼屈光度比较差异无统计学意义,平均差值(0．19±0．92)D；B组左右眼眼轴比较差异无统计学意义,平均差值(0．15±0．50)mm；去遮盖眼后极部巩膜中的MMP-2含量明显下降,与对照组之间差异无统计学意义(P=0．47)。结论形觉剥夺性近视眼的小鸡模型中,近视眼的形成与后巩膜中的MMP-2水平有关,而且MMP-2的含量在近视眼发展期和恢复期存在一定的变化规律。(眼科,2006,15：334-337)
OBJECTIVE: To establish a model of myopic eye in rats and observe the changes of refractive status and the content of matrix metalloproteinase (MMP-2) during the development and recovery of myopia, and to explore the biochemical changes in scleral remodeling of myopic chick model mechanism. Design Randomized controlled experimental study. Study objects 32 2-day-old SPF United States imported white Lai Lai chicken. Methods Thirty-two chickens were randomly divided into four groups (A, B, C and D), with 8 rats in each group. Groups A and B were experimental groups. The eyes of the chickens were covered with a transparent eye mask and visual deprived by one eye. Control; C, D group as a normal control. After 21 days, the eyes were examined for refractive status and axial length of the eyes. A and C groups were sacrificed and 7 mm diameter posterior scleral tissues were removed. Groups B and D were continued until 21 days after de-masking, and the refractive status and eyes Axis was sacrificed and a 7 mm diameter posterior scleral tissue was isolated. The content of MMP-2 in sclera of all animals was determined by enzyme activity assay, and the data of experimental group and control group were statistically analyzed. The main index After covering and to cover the chickens after diopter, axial, sclera MMP-2 content. Results There was significant difference between the left and right eyes in group A and group B (P = 0.000) for 21 days, with an average difference of (-28.38 ± 6.72) D. (P = 0.000), with a mean difference of (1.47 ± 0.37) mm. The content of MMP-2 in the sclera of the posterior pole of the eye was significantly increased, while the occlusion of the eye in the group A was unobscured The difference between the eyes was statistically significant, with an average increase (76.00 ± 15.20)%. There was no significant difference in diopter between the left and right eyes of Group B in 21 days (mean, 0.19 ± 0.92) D; there was no significant difference between the two groups (P> 0.05) ± 0.50) mm. The content of MMP-2 in the sclera of the posterior pole of the retina was significantly lower than that of the control group (P = 0.47). Conclusions In myopic deprivation myopia chick model, the formation of myopia is related to the level of MMP-2 in posterior sclera, and the content of MMP-2 in myopia development and recovery period has some changes. (Ophthalmology, 2006,15: 334-337)