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目的 研究弓形虫与宿主细胞之间相互作用的钙通道机制。 方法 以急性期感染小鼠腹水收集的RH株刚地弓形虫速殖子为材料 ,采用荧光染料 Fura- 2与细胞质游离钙结合 ,通过荧光分光光度计技术和 SuperIon Probe Software计算机软件测定弓形虫速殖子细胞质游离钙浓度 ([Ca2 + ]i)。 结果 正常弓形虫速殖子[Ca2 + ]i为 (2 0 5 .0 2± 18.6 ) nm ol/ L,符合文献报道的静息状态下真核细胞 [Ca2 + ]i浓度范围。 结论 本方法简便、易行 ,相对费用低 ,适用于国内实验室
Objective To study the calcium channel mechanism of interaction between Toxoplasma gondii and host cells. Methods Toxoplasma gondii RH strain Strain RH was collected from the ascites of mouse ascites infected by acute phase. The fluorescent dye Fura-2 was combined with cytoplasmic free calcium to determine Toxoplasma gondii by fluorescence spectrophotometer and SuperIon Probe Software Cytoplasmic free calcium concentration ([Ca2 +] i). Results The tachyzoite [Ca2 +] i of normal Toxoplasma gondii was (20 05 ± 18.6) nm ol / L, which accorded with the concentration range of [Ca2 +] i in resting eukaryotic cells reported in the literature. Conclusion This method is simple, easy to operate, relatively low cost, suitable for domestic laboratories