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目的:测定思茅松不同部位莽草酸的含量。方法:采用高效液相色谱法,色谱柱为Agilent zorbax-C18(4.6 mm×150 mm,5μm),流动相甲醇-1%磷酸水溶液(3∶97),流速0.8 mL.min-1,检测波长213 nm,柱温25℃。结果:莽草酸在0.01～0.8μg线性相关良好(r=0.999 9),平均加样回收率为99.00%。RSD 0.81%(n=9)。结论:不同部位思茅松中莽草酸的含量存在显著差异,松针中莽草酸含量最高为29.17 mg.g-1,可以考虑将松针作为新的莽草酸资源加以开发利用。
Objective: To determine the content of shikimic acid in different parts of Pinus kesi Methods: High performance liquid chromatography with Agilent zorbax-C18 column (4.6 mm × 150 mm, 5 μm), mobile phase methanol-1% phosphoric acid solution (3:97), flow rate 0.8 mL.min-1, 213 nm, column temperature 25 ℃. Results: Shikimic acid had a good linear correlation at 0.01-0.8 μg (r = 0.999 9) with an average recovery of 99.00%. RSD 0.81% (n = 9). CONCLUSIONS: The contents of shikimic acid in different parts of Simao pine were significantly different. The highest content of shikimic acid in pine needles was 29.17 mg.g-1. Consideration could be given to the development and utilization of pine needles as a new shikimic acid source.