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以培养的恶性疟原虫ＮＦ５４（３Ｄ７）株配子体蛋白抽提液及我国云南现场采集的恶性疟原虫细胞骨架分别免疫ＢＡＬＢ／ｃ小鼠。取免疫小鼠脾细胞与ＳＰ２／０骨髓瘤细胞融合，以ＩＦＡ法筛选出８株抗恶性疟原虫有性期ＭｃＡｂ杂交瘤细胞株。经免疫球蛋白类别鉴定，６株为ＩｇＧ１（Ｍ２Ａ１０Ｃ９、Ｍ２Ｃ１Ｂ８、Ｍ４Ｃ７Ｂ１０、Ｍ４Ｇ１２Ｃ１、Ｍ５Ｂ７Ｅ６和Ｍ６Ｅ１Ｇ１１），２株为ＩｇＭ（Ｍ４Ｄ７Ｆ７和Ｍ６Ｆ４Ｄ６）。其中３株ＭｃＡｂｓ（Ｍ４Ｃ７Ｂ１０、Ｍ４Ｄ７Ｆ７和Ｍ６Ｅ１Ｇ１１）的靶抗原定位于配子体以及大滋养体和裂殖体期无性体原虫；其余５株仅定位于配子体。经Ｗｅｓｔｅｒｎ印迹试验，ＭｃＡｂ所识别的蛋白区带各异（１６－１２０ｋＤ），与已发现的有性期特异性抗原相比较，３２ｋＤ抗原国内外尚未报道。各株ＭｃＡｂ与猴疟（Ｐ．ｃｙｎｏｍｏｌｇｉ）红内期、鸡疟（Ｐ．ｇａｌｉｎａｃｅｕｍ）子孢子和杜氏利什曼原虫前鞭毛体均无交叉反应。
BALB / c mice were respectively immunized with the extract of gametophyte protein of Plasmodium falciparum NF54 (3D7) and the cytoskeleton of Plasmodium falciparum collected from Yunnan in China. The splenocytes of immunized mice were fused with SP2 / 0 myeloma cells, and 8 hybridoma cell lines with sexual stage of Plasmodium falciparum were screened by IFA. Of the 6 isolates, IgG1 (M2A10C9, M2C1B8, M4C7B10, M4G12C1, M5B7E6 and M6E1G11) were identified by immunoglobulin class and 2 strains were IgM (M4D7F7 and M6F4D6). The target antigens of three McAbs (M4C7B10, M4D7F7 and M6E1G11) were located in gametophytes, as well as in trophozoites and merozoites of meiosis stage. The other 5 strains were only located in gametophytes. After Western blotting, McAb recognized different protein bands (16-120kD), compared with the found sex-specific antigen, 32kD antigen has not been reported at home and abroad. McAb of each strain did not cross-react with P. cygnomgi within the red stage, P. galinaceum sporozoites and Leishmania donovani promastigotes.