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血小板生长因子（ｔｈｒｏｍｂｏｐｏｉｅｔｉｎ，ＴＰＯ）是一种正常生理状态下的造血生长因子，它刺激造血祖细胞向巨核细胞的分化及血小板的生成和释放。该实验从６月龄人胚肝脏中分离ＲＮＡ，经逆转录ＰＣＲ等步骤克隆出了ＴＰＯｃＤＮＡ，并进行了序列分析。将ＴＰＯｃＤＮＡ亚克隆至哺乳动物细胞瞬时表达载体ｐＣＭＶ４，形成了重组表达质粒ｐＣＭＶ４／ＴＰＯ。以该重组质粒转染ＣＯＳ７细胞，可测到ＴＰＯ在ＣＯＳ７细胞中的瞬时表达
Thrombopoietin (TPO) is a normal physiological state of hematopoietic growth factor that stimulates the differentiation of hematopoietic progenitor cells into megakaryocytes and the production and release of platelets. In this experiment, RNA was isolated from 6-month-old human embryonic liver. The TPO cDNA was cloned by reverse transcription PCR and sequenced. The TPO cDNA was subcloned into mammalian cell transient expression vector pCMV4 to form the recombinant expression plasmid pCMV4 / TPO. Transfection of COS7 cells with the recombinant plasmids could detect the transient expression of TPO in COS7 cells