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结果表明:1.在粘、易型胞质背景下,产生单倍体的主要遗传机理是因为n型1B/1R卵细胞未受精前由于与粘质或易质的专一相互作用,卵核提前分裂而导致孤雌生殖的结果;2.一般从1B·1B/1R杂合核型形成的n型1B/1R卵核要比来自1B/1R·1B/1R纯合型的卵核产生较高频率的单倍体,纯合1B/1R·1B/1R核型世代间有着相对趋于稳定的单倍体诱导频率;3.在同核背景下,诱导单倍体频率粘质高于易质;4.来源不同的1B/1R易位系,1R短臂确实存在着整臂易位和部分小片段易位间的区别,据此差异进行亲本选择,可以选出不产生或很少产生单倍体的粘、易型1B/1R不育系。此外,分析了粘、易型1B/1R不育系一般恢复度不高的机理,初步认为可能与1B·1B/1R杂合易位染色体在减数分裂中能否正常联会配对有直接关系。
The results showed as follows: 1. The main genetic mechanism of haploid generation in the viscous and eosinophilic cytoplasm was that the egg nucleus was advanced due to the specific interaction with the nidus or perishable before the n-type 1B / 1R egg cells were not fertilized Split and lead to parthenogenesis results; 2. The n-type 1B / 1R egg nuclei generally formed from 1B · 1B / 1R hybrid karyotypes produced higher than egg nuclei from 1B / 1R · 1B / 1R homozygotes Frequency haploid and homozygous 1B / 1R · 1B / 1R karyotypes had relatively stable frequency of haploid induction.3. In the same nuclear background, the haploid frequency induced higher than the susceptible ; 4. Different 1B / 1R translocation lines from different origins, 1R short arm does exist the difference between whole arm translocation and partial small fragment translocation. Based on the difference, Ploidy sticky, easy type 1B / 1R CMS. In addition, the mechanism of low recovery rate of 1B / 1R male sterile line 1B / 1R was analyzed. The possible reason is that 1B / 1B / 1R heterozygous translocation chromosome may be directly associated with normal pairing in meiosis .