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目的探讨传染性非典型肺炎(急性重症呼吸综合征,SARS)患者外周血CD4+T细胞CD38的表达的变化及其与病程和病情严重程度的关系。方法用双色流式细胞术测定157例SARS临床诊断病例、37例同期发生的肺炎支原体感染患者及38名健康成人外周血淋巴细胞中CD4+T细胞的百分率(CD4%)、CD4+T细胞CD38阳性率(CD38/CD4%)及CD38平均荧光强度(CD38MF)进行检测。结果SARS病例CD4%与健康对照组相比均无统计学意义,但其CD38/CD4%及CD4+T细胞CD38MF显著低于健康对照组(P<0.05,P<0.05);SARS重症患者CD38/CD4%及CD38MF显著低于轻症患,有统计学意义(P<0.01,P<0.001);SARS轻症患者CD4%、CD38/CD4%及CD38MF与健康对照组相比无统计学意义;SARS临床诊断病例CD4%显著高于肺炎支原体感染者,有统计学意义(P<0.01),而CD38/CD4%及CD38MF显著低于肺炎支原体感染,有统计学意义(P<0.01,P<0.01)。结论SARS患者CD4+T细胞CD38的表达降低,降低的水平与SARS病情严重程度相关。CD4+T细胞CD38的表达在SARS诊断病例及肺炎支原体感染中的变化存在统计学意义,CD4+T细胞CD38的表达的检测有助于SARS与肺炎支原体感染的鉴别。
Objective To investigate the changes of CD38 expression in CD4 + T cells in peripheral blood of patients with SARS (acute severe respiratory syndrome) and its relationship with the course of disease and the severity of the disease. Methods The percentage of CD4 + T cells in peripheral blood lymphocytes of 157 patients with SARS and 37 patients with mycoplasma pneumoniae infection in the same period and 38 healthy adults were measured by two-color flow cytometry. CD4 + T cells CD38 Positive rate (CD38 / CD4%) and CD38 average fluorescence intensity (CD38MF) were detected. Results CD4% of SARS cases were not significantly different from healthy control group, but CD38 / CD4% and CD38MF of CD4 + T cells in SARS patients were significantly lower than those in healthy controls (P <0.05, P <0.05) CD4% and CD38MF were significantly lower than those in mild cases (P <0.01, P <0.001). There was no significant difference in CD4%, CD38 / CD4% and CD38MF between SARS patients and controls CD4% in clinical diagnosis was significantly higher than that in Mycoplasma pneumoniae (P <0.01), while CD38 / CD4% and CD38MF were significantly lower than those in Mycoplasma pneumoniae (P <0.01, P <0.01) . Conclusions The expression of CD38 on CD4 + T cells in SARS patients is decreased. The level of CD38 is correlated with the severity of SARS. CD38 expression of CD4 + T cells in SARS cases and mycoplasma pneumoniae infection in patients with changes in the statistical significance of CD34 + CD4 + T cells in the detection of help to identify SARS and Mycoplasma pneumoniae infection.