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目的:研究前列腺癌及BPH组织中PSA和增殖细胞核抗原(PCNA)表达的关系及其临床意义。方法:应用免疫组织化学LDP法检测76例前列腺癌(PCa)、40例BPH组织中PSA及PCNA的表达情况。结果:①对PSA染色,大多数前列腺癌表达低(62/76),少数分化好的癌组织呈阳性表达(14/76);而BPH组织中,PSA呈高表达,两者比较差异有统计学意义(P<0.01);随着组织学分级及临床分期的增加,前列腺癌PSA表达下调(P<0.01);PSA表达与血清PSA水平差异无统计学意义(P>0.05)。②PCNA在前列腺癌组织中表达较BPH组织增加,差异有统计学意义(P<0.01);PCNA的表达随着组织学分级及临床分期的增加而增加(P<0.01);PCNA高表达组(3、4级)术后生存率明显低于低表达组(1、2级)(P<0.05)。③PSA与PCNA的表达具有明显的负相关性(P<0.01)。结论:PSA和PCNA的表达与前列腺癌的发生、发展有一定的关系;二者结合对其肿瘤的恶性程度、生物学行为及预后的判断更为准确。
Objective: To investigate the relationship between the expression of PSA and proliferating cell nuclear antigen (PCNA) in prostate cancer and BPH and its clinical significance. Methods: The expressions of PSA and PCNA in 76 cases of prostate cancer (PCa) and 40 cases of BPH were detected by immunohistochemical LDP method. Results: (1) For PSA staining, most of the prostate cancers had a low expression (62/76), and a few well-differentiated cancerous tissues were positive (14/76). In BPH tissues, PSA was highly expressed, with statistical difference (P <0.01). With the increase of histological grade and clinical stage, PSA expression of prostate cancer was down-regulated (P <0.01). There was no significant difference between PSA expression and serum PSA level (P> 0.05). The expression of PCNA in prostate cancer tissues was significantly higher than that in BPH tissues (P <0.01). The expression of PCNA increased with the histological grade and clinical stage (P <0.01) , Grade 4) postoperative survival rate was significantly lower than the low expression group (1,2 grade) (P <0.05). ③PSA and PCNA expression had a significant negative correlation (P <0.01). Conclusions: The expression of PSA and PCNA has a certain relationship with the occurrence and development of prostate cancer. The combination of the two can be more accurate in judging the malignant degree, biological behavior and prognosis of the patients.