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目的探讨高碘对大鼠下腔静脉肝段内皮细胞活化的影响。方法选取Wistar大鼠40只,随机分成四组,各10只。给予对照A组碘含量为300μg/kg的正常饲料和碘含量为5μg/L自来水;给予对照B组碘含量为300μg/kg的偏食饲料和碘含量为5μg/L自来水;给予观察A组碘含量为300μg/kg的偏食饲料和碘含量10050μg/L的碘溶液;给予观察B组碘含量为300μg/kg的偏食饲料和碘含量20300μg/L的碘溶液。四组大鼠连续喂养24周,每周对四组大鼠进行称重。ELISA观察大鼠下腔静脉肝段内皮细胞活化情况。结果在第6、12、18、24周,对照B组、观察A组、观察B组的体重明显低于对照A组,差异均有统计学意义(P<0.05);观察A组和观察B组的vWFpp、vWF、ICAM-1含量和对照A组比较,差异均有统计学意义(P<0.05)。结论高碘摄入会造成大鼠下腔静脉肝段内皮细胞活化,偏食会加重高碘摄入所造成大鼠下腔静脉肝段内皮细胞活化。
Objective To investigate the effect of high iodine on the activation of endothelial cells in the hepatic segment of inferior vena cava in rats. Methods Forty Wistar rats were randomly divided into four groups with 10 rats each. The control group A had a normal diet with an iodine content of 300 μg / kg and an iodine content of 5 μg / L of tap water. In the control group B, partial egestin with an iodine content of 300 μg / kg and iodine content of 5 μg / Which were 300g / kg partial eclipse feed and 10050μg / L iodine solution; Group B was given partial eclipse feed with iodine content of 300μg / kg and iodine solution with iodine content of 20300μg / L. Four groups of rats were fed continuously for 24 weeks, and four groups of rats were weighed weekly. ELISA was used to observe the activation of endothelial cells in the hepatic segment of rat inferior vena cava. Results At the 6th, 12th, 18th and 24th week, the body weight of the control group B, the observation group A and the observation group B were significantly lower than those of the control group A, the differences were statistically significant (P <0.05) The levels of vWFpp, vWF, ICAM-1 in group A and group A were significantly different (P <0.05). Conclusion High iodine intake can cause endothelial cell activation in the hepatic segment of rat inferior vena cava, and partial eclipse may aggravate the activation of endothelial cells in the hepatic segment of rat inferior vena cava induced by high iodine intake.