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目的观察辅酶Q1(0Coenzyme Q10,CoQ10)对中波紫外线(Ultraviolet radiation B,UVB)致小鼠成纤维细胞NIH3T3损伤的拮抗作用。方法采用血清药理学方法制备含有及不含有CoQ10的血清;将NIH3T3细胞接种于12孔培养板,分别接受不同剂量UVB(0、10、30、60和90 mJ/cm2)照射,MTT法检测细胞增殖活力;分别加入10、20和40μl含药血清,并设空白对照组和不含药对照血清组,经UVB照射后,MTT法检测细胞增殖活力;取细胞培养上清液,使用丙二醛(MDA)试剂盒测定MDA的含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)试剂盒分别测定SOD、CAT和GSH-Px的活性。结果经大剂量(30、60、90 mJ/cm2)UVB照射,NIH3T3细胞增殖活力明显降低(P<0.01),UVB照射后,40μl含药血清组较不含药对照血清组细胞增殖活力明显增强(P<0.05),不含药对照血清组与空白对照组相比,MDA含量明显升高,SOD、GSH-Px和CAT的活性明显降低(P<0.05);40μl含药血清组与不含药对照血清组相比,MDA含量明显降低,SOD和GSH-Px的活性明显升高(P<0.05);但CAT活性差异无统计学意义(P>0.05)。结论 CoQ10能够显著的拮抗UVB照射所引起的小鼠成纤维细胞氧化损伤。
Objective To observe the antagonism of coenzyme Q10 (CoQ10) on NIH3T3 injury induced by UVB in mouse fibroblasts. Methods Serum pharmacological methods were used to prepare serum containing CoQ10. NIH3T3 cells were seeded on 12-well plates and irradiated with different doses of UVB (0, 10, 30, 60 and 90 mJ / cm2), respectively. Proliferative activity; 10, 20 and 40μl drug-containing serum were added respectively, and the blank control group and control drug-free serum group were set up. After UVB irradiation, cell proliferation activity was detected by MTT assay; cell culture supernatant was treated with malondialdehyde MDA, SOD, CAT and GSH-Px were measured to determine the levels of MDA, SOD, CAT and GSH- Px activity. Results The proliferative activity of NIH3T3 cells was significantly decreased (P <0.01) by high dose (30,60 and 90 mJ / cm2) UVB irradiation. After UVB irradiation, the proliferation activity of 40μ1 serum group was significantly higher than that of the control serum group (P <0.05). Compared with the blank control group, the content of MDA, the activity of SOD, GSH-Px and CAT in the serum without drug control group were significantly decreased (P <0.05) Compared with the control group, the content of MDA was significantly decreased and the activities of SOD and GSH-Px were significantly increased (P <0.05). However, there was no significant difference in CAT activity between the two groups (P> 0.05). Conclusion CoQ10 can significantly antagonize the oxidative damage induced by UVB in mouse fibroblasts.