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目的探讨呼出气冷凝液(EBC)中胃泌素释放肽前体(Pro GRP)在小细胞肺癌(SCLC)患者诊断中的应用。方法收集SCLC患者30例,局限期14例,广泛期16例;NSCLC患者41例,鳞癌20例,腺癌21例;同期健康体检者30例为对照组。采集空腹血清和呼出气冷凝液(EBC)标本,采用ELISA方法检测血清及EBC中Pro GRP。结果 SCLC组血清中Pro GRP水平高于NSCLC组和对照组,差异有统计学意义(P<0.05)。SCLC组EBC中Pro GRP水平高于NSCLC组和对照组,差异有统计学意义(P<0.05)。广泛期血清Pro GRP水平高于局限期,差异有统计学意义(P<0.05);广泛期EBC中Pro GRP水平与局限期比较差异无统计学意义(P>0.05);局限期血清和EBC中Pro GRP水平均高于对照组,差异有统计学意义(P<0.05)。结论检测血清及EBC中Pro GRP水平对肺癌有辅助诊断价值,其中EBC中Pro GRP检测可作为SCLC早期诊断的无创指标。
Objective To investigate the application of gastrin-releasing peptide precursor (Pro GRP) in the diagnosis of small cell lung cancer (SCLC) in exhaled breath condensate (EBC). Methods Thirty patients with SCLC were recruited, including 14 patients in a limited period and 16 patients in a wide-range. There were 41 patients with NSCLC, 20 with squamous cell carcinoma and 21 with adenocarcinoma. Thirty healthy controls were included as control group. Fasting serum and exhaled breath condensate (EBC) specimens were collected, and serum and ProBC in EBC were detected by ELISA. Results The serum level of Pro GRP in SCLC group was significantly higher than that in NSCLC group and control group (P <0.05). The level of Pro GRP in EBC of SCLC group was higher than NSCLC group and control group (P <0.05). The Pro GRP level in the extensive stage was higher than that in the limited stage (P <0.05). There was no significant difference in Pro GRP level between the extended stage and the limited stage (P> 0.05). In the limited stage serum and EBC Pro GRP levels were higher than the control group, the difference was statistically significant (P <0.05). Conclusions Detection of Pro GRP levels in serum and EBC may be of value in the diagnosis of lung cancer. The detection of Pro GRP in EBC can be used as a noninvasive indicator of early diagnosis of SCLC.