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本文应用3H-胸腺嘧啶核苷(3H-thymidine,3H-TdR)掺入法及3H-脯氨酸(3H-proline,3H-Pro)掺入法观察白细胞介素1β(interleukin-1β,IL-1β)对Spague-Dawley乳鼠心肌成纤维细胞DNA及胶原合成的影响,并用明胶酶谱法和Western blot检测基质金属蛋白酶(matrix metalloproteinases,MMPs)MMP-2、MMP-9活性及MMP-2和MMP-9蛋白表达,用RT-PCR检测MMP-2、MMP-9的mRNA表达。结果显示:(1)0.1、1、10、100ng/mL的IL-1β作用于细胞24h后,各组3H-TdR掺入量明显较对照组低(P<0.05,P<0.01),同时3H-Pro掺入量明显降低(P<0.05,P<0.01);而0.01ng/mL的IL-1β作用于细胞后,对3H-TdR掺入量和3H-Pro掺入量无明显影响。(2)不同剂量(0.01~100ng/mL)的IL-1β均刺激MMP-2和MMP-9活性升高,并呈剂量依赖性。IL-1β增加MMP-2和MMP-9蛋白表达(P<0.05,P<0.01)。(3)IL-1β(0.01~100ng/mL)刺激MMP-2和MMP-9mRNA表达升高(P<0.05,P<0.01)。以上结果表明,IL-1β通过减少心肌成纤维细胞的细胞分裂来降低胶原的合成,同时促进MMP-2和MMP-9的转录及转录后的表达来促进胶原的分解,提示其在心肌重塑过程中起一定作用。
In this study, the effects of interleukin-1β (IL-1β) and interleukin-1β (IL-1β) 1β) on the DNA synthesis and collagen synthesis in myocardial fibroblasts of Spague-Dawley rats. The activities of MMP-2, MMP-9 and MMP-2 and MMP-9 in matrix metalloproteinases (MMPs) MMP-9 protein expression, RT-PCR detection of MMP-2, MMP-9 mRNA expression. The results showed that: (1) After treated with 0.1, 1, 10 and 100ng / mL of IL-1β for 24h, the 3H-TdR incorporation in each group was significantly lower than that in the control group (P <0.05, P <0.01) -Pro incorporation decreased significantly (P <0.05, P <0.01). However, 0.01ng / mL IL-1β had no effect on 3H-TdR incorporation and 3H-Pro incorporation. (2) IL-1βin different doses (0.01-100ng / mL) stimulated the activity of MMP-2 and MMP-9 in a dose-dependent manner. IL-1β increased the expression of MMP-2 and MMP-9 (P <0.05, P <0.01). (3) IL-1β (0.01-100ng / mL) stimulated the expression of MMP-2 and MMP-9mRNA (P <0.05, P <0.01). The above results indicate that IL-1β can promote the decomposition of collagen by decreasing the cell division of cardiac fibroblasts, reducing the synthesis of collagen, and promoting the transcription and transcription of MMP-2 and MMP-9, Play a role in the process.