Lactase non-persistence and milk consumption in Estonia

• 作 者: Margus Lember Suvi Torniainen Mart Kull Riina Kallikorm Peeter Saadla Tarvo Rajasalu Hanna Komu Irma Jrvel
• 机 构: Department of Internal Medicine University of Tartu Tartu Estonia ,Department ofMedical Genetics Un
• 出 处: World Journal of Gastroenterology
• 发表日期: 2006年45期
• 关键词: persistence intolerance genotype upstream allele Finland sequencing facilitating

AIM: To defi ne the frequency of the C/T-13910 variant associated with lactase persistence/non-persistence trait and to analyze the milk consumption of lactase non- persistent subjects in Estonia. METHODS: We genotyped 355 Estonians by polymerase chain reaction and direct sequencing. Milk consumption was analyzed by a questionnaire, specially developed to analyze milk consumption and abdominal complaints. RESULTS: The frequency of the genotype of the C/ C-13910 (lactase non-persistence) was found to be 24.8% in native Estonians. No other single nucleotide polymorphisms covering the region of 400 bp adjacent to the C/T-13910 variant were found. Lactase non- persistence subjects were found to consume less milk than lactase persistence subjects. CONCLUSION: The frequency of lactase non- persistence defi ned by the C/C-13910 genotype confi rms the results of the previous studies based on indirect methods of determining hypolactasia. Milk consumption of lactase non-persistence subjects is consistent with previously reported figures of adult-type hypolactasia in Estonia. However, lactase non-persistence does not prevent the intake of milk in many adults.

AIM: To defi ne the frequency of the C / T-13910 variant associated with lactase persistence / non-persistence trait and to analyze the milk consumption of lactase non- persistent subjects in Estonia. METHODS: We genotyped 355 Estonians by polymerase chain reaction and direct sequencing. Milk consumption was analyzed by a questionnaire, specially developed to analyze milk consumption and abdominal complaints. RESULTS: The frequency of the genotype of the C / C-13910 (lactase non-persistence) was found to be 24.8% in native Estonians . No other single nucleotide polymorphisms covering the region of 400 bp adjacent to the C / T-13910 variant were found. Lactase non-persistence subjects were found to consume less milk than lactase persistence subjects. CONCLUSION: The frequency of lactase non- persistence defi ned by the C / C-13910 genotype confi rms the results of the previous studies based on indirect methods of determining hypolactasia. Milk consumption of lactase non-persistence subjects is c onsistent with previously reported figures of adult-type hypolactasia in Estonia. However, lactase non-persistence does not prevent the intake of milk in many adults.