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目的:探讨辛伐他汀(SIM)对雷帕霉素(RAPA)作用下大鼠心肌微血管内皮细胞(CMECs)增殖、迁移、凋亡及一氧化氮(NO)分泌的影响。方法:用不同浓度(0、0.01、0.1、1及10μg/L)的RAPA处理CMECs 24 h,分别采用MTT比色法及transwell法检测细胞的增殖能力和迁移能力,并选择合适干扰浓度(抑制效果居中,即对细胞的增殖及迁移有一定的抑制作用,但不会完全抑制,选择浓度为1μg/L)。对已加入RAPA(1μg/L)的细胞中加入不同浓度(0、0.01、0.1、1及10μmol/L)的SIM,共孵育24 h,采用MTT比色法检测细胞的增殖能力,用transwell法检测细胞迁移能力,用Hoechst染色法计算细胞的凋亡率,用Griess反应检测NO的分泌活性。结果:与对照组相比较,单纯以RAPA干预细胞后,细胞增殖和迁移的能力均显著下降(P<0.05,P<0.01),且呈浓度依赖性。而在RAPA基础上加入不同浓度的SIM后,与单纯RAPA组比较,细胞的增殖能力及迁移能力均显著增强(P<0.05,P<0.01),NO的分泌活性明显升高(P<0.05),细胞的凋亡率明显下降(P<0.05)。结论:RAPA能抑制CMECs增殖及迁移、NO分泌并诱导其凋亡。而将CMECs与SIM共孵育24 h后,对RAPA诱导的细胞损伤具有抑制作用。
Objective: To investigate the effects of simvastatin on proliferation, migration, apoptosis and nitric oxide (NO) secretion of rat cardiac microvascular endothelial cells (CMECs) induced by rapamycin (RAPA). Methods: The CMECs were treated with RAPA at different concentrations (0, 0.01, 0.1, 1 and 10 μg / L) for 24 h. The proliferation and migration ability of CMECs were determined by MTT assay and transwell assay respectively. The effect of the middle, that is, the proliferation and migration of cells have a certain inhibitory effect, but not completely inhibited, the choice of concentration of 1μg / L). Cells of different concentrations (0, 0.01, 0.1, 1 and 10 μmol / L) were added into RAPA (1 μg / L) cells and incubated for 24 h. The migration ability of cells was detected. The apoptosis rate of cells was calculated by Hoechst staining. The secretion activity of NO was detected by Griess reaction. Results: Compared with the control group, the ability of cell proliferation and migration after RAPA intervention was significantly decreased (P <0.05, P <0.01), and in a concentration-dependent manner. Compared with RAPA group, the proliferation and migration ability of cells increased significantly (P <0.05, P <0.01) and the secretion activity of NO significantly increased (P <0.05) , The apoptosis rate of cells decreased significantly (P <0.05). Conclusion: RAPA can inhibit the proliferation and migration of CMECs, the secretion of NO and the induction of apoptosis. However, when incubated with SIMs for 24 h, CMECs could inhibit RAPA-induced cell injury.