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目的观察孤独症大鼠突触相关蛋白突触素(SYN)、突触后致密蛋白-95(PSD-95)、桥尾蛋白(gephyrin)在前额叶皮质的表达变化,探讨突触相关蛋白在孤独症发病中的作用。方法采用孕12.5d(E12.5)一次性腹腔注射丙戊酸钠(600mg/kg)的孕鼠所产下的子代鼠作为模型组,同样方法注射同等剂量的生理盐水的孕鼠所产下子代鼠作为正常组。通过斜板实验、睁眼实验、三箱实验验证模型是否成功;Western blotting方法对比生后42天两组大鼠突触相关蛋白SYN、PSD-95、gephyrin在前额叶皮质的表达变化。结果 1)成功建立孤独症动物模型:与正常组比较,模型组大鼠生长发育迟缓;社会交往能力异常、对新鲜事物偏好障碍,差异均有统计学意义(P均<0.05);2)Western blotting结果显示:与正常组比较,孤独症大鼠突触相关蛋白SYN、PSD-95表达显著增多(P<0.05),gephyrin蛋白表达显著减少(P<0.05)。结论孤独症大鼠前额叶皮质突触蛋白表达异常。
Objective To observe the changes of the expression of synaptophysin (SYN), postsynaptic density-95 (PSD-95) and gephyrin in the prefrontal cortex of autistic rats and to explore the relationship between synaptic-related protein The role of autism in the pathogenesis. Methods The offspring of pregnant rats born of intraperitoneal injection of sodium valproate (600mg / kg) 12.5d (E12.5) were used as the model group, and the same method of injecting the same dose of saline Next generation offspring as a normal group. The experiment was conducted by oblique plate experiment, eyes open experiment and three boxes experiment to verify whether the model was successful. Western blotting method was used to compare the expression of SYN, PSD-95 and gephyrin in prefrontal cortex of 42 days after birth. Results 1) Animal model of autism was established successfully. Compared with the normal group, the rats in the model group grew slowly and stunted; the ability of social intercourse was abnormal and the preferences of new things were obstructive. The differences were statistically significant (all P <0.05); 2) Western The results of blotting showed that compared with the normal group, the expression of SYN, PSD-95 and the gephyrin protein in autism rats were significantly increased (P <0.05). Conclusion Autonomic rat prefrontal cortex synaptophysin expression is abnormal.