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目的研究欧前胡素在大鼠血浆、肝脏中的药动学。方法大鼠单剂量灌胃给予欧前胡素后,采用HPLC法测定不同时间内大鼠血浆、肝脏中的欧前胡素。色谱柱为Phenomenex Gemini C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(65∶35,V/V),UV检测器,内标为醋酸氟轻松。结果欧前胡素血浆的t1/2(β)=5.893 h,Tmax=0.667 h,Cmax=5.026 mg.L-1,V1为28.238 mL.kg-1,CL为3.3212 mL.h-1.kg-1,AUC0-t为13.947 h.μg.mL-1,AUC0-∞为18.246 h.μg.mL-1;欧前胡素肝脏组织t1/2(β)=5.935 h,Tmax=0.667 h,Cmax=1.2μg.g-1,V1为112.512 mL.kg-1,CL为12.893 mL.h-1.kg-1,AUC0-t为3.678 h.μg.mL-1,AUC0-∞为4.700 h.μg.mL-1;结论欧前胡素口服给药后迅速进入血液、肝脏,消除也较快。
Objective To study the pharmacokinetics of imperatorin in rat plasma and liver. Methods Imperatorin was administered to rats’ plasma and liver in different time by HPLC after single dose of imperatorin. The chromatographic column was Phenomenex Gemini C18 column (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-water (65:35, V / V) UV detector with fluocinolone acetonide. Results Imperatorin plasma t1 / 2 (β) = 5.893 h, Tmax = 0.667 h, Cmax = 5.026 mg.L-1, V1 was 28.238 mL.kg-1, CL was 3.3212 mL.h.lkg 1, the AUC0-t was 13.947 h.μg.mL-1, the AUC0-∞ was 18.246 h.μg.mL-1; the t1 / 2 (β) of allotulin was 5.935 h and the Tmax was 0.667 h Cmax = 1.2 μg.g-1, V1 was 112.512 mL.kg-1, CL was 12.893 mL.h-1.kg-1, AUC0-t was 3.678 h.μg.mL-1 and AUC0-∞ was 4.700 h .μg.mL-1; Conclusion Imperatorin oral administration quickly into the blood, liver, eliminate faster.