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用呼吸电子传递细胞色素途径的抑制剂氰化钾（ＫＣＮ）与抗氰呼吸途径的抑制剂水杨基氧肟酸（ＳＨＡＭ）处理水稻细胞质雄性不育系（ＣＭＳ）珍汕９７Ａ及其保持系珍汕９７Ｂ的幼穗和花药后，ＫＣＮ使不育系与保持系的超氧阴离子自由基（Ｏ２■）产生受到抑制，不育系的Ｏ２■的形成受抑制较多。ＳＨＡＭ处理则增高Ｏ２■形成，以不育系的增加较多．ＫＣＮ与ＳＨＡＭ处理后都使不育系与保持系的丙二醛（ＭＤＡ）含量升高，ＫＣＮ使保持系的ＭＤＡ含量升高较多，ＳＨＡＭ则使不育系的ＭＤＡ含量升高较多．ＫＣＮ处理后，不育系与保持系的超氧物歧化酶（ＳＯＤ）活性下降，ＳＨＡＭ处理后不育系与保持系的ＳＯＤ活性变化不明显。Ｈ２Ｏ２处理对不育系与保持系幼穗的呼吸速率影响不大．Ｈ２Ｏ２＋ＦｅＳＯ４处理后，使呼吸速率大幅度下降，表明Ｈ２Ｏ２＋ＦｅＳＯ４所形成的羟自由基（ＯＨ）比Ｈ２Ｏ２对呼吸代谢的破坏作用更大。
Treatment of Rice Cytoplasmic Male Sterile CMS (CMS) Zhenshan 97A and Its Maintaining System by Inhibitors of Cytochrome Pathway Using Respiratory Electron Transporter KCN and Inhibitors of Cyanide Respiratory Pathway Salicylic Acid Hydroxamic Acid (SHAM) After young panicle and anther of Zhenshan 97B, KCN inhibited the production of superoxide anion radical (O2 ■) in CMS line and maintainer line, and suppressed the formation of O2 ■ in CMS line. SHAM treatment increased O2 ■ formed to increase more sterile lines. Both KCN and SHAM increased the content of malondialdehyde (MDA) in CMS and maintainer lines, KCN increased the content of MDA in maintainer lines and SHAM increased the content of MDA in CMS lines. After KCN treatment, the activity of superoxide dismutase (SOD) in CMS and maintainer lines decreased, while SOD activity in CMS lines and maintainer lines did not change significantly after SHAM treatment. H2O2 had little effect on the respiration rate of spikelets in sterile and maintainer lines. H2O2 + FeSO4 treatment, the respiration rate decreased significantly, indicating that the H2O2 + FeSO4 hydroxyl radical (OH) than H2O2 on the respiratory metabolism of the greater role.