论文部分内容阅读
目的通过对2014-2015年北辰区疾病预防控制中心实验室对应的哨点医院-天津市第四中心医院采集流感样的病原学监测,明确2014-2015年流感流行株,毒株变异情况,为流感的疫情发展及控制提供科学的依据。方法对疑似流感患者进行咽拭子标本采集后做核酸检测,对阳性标本用MDCK进行毒株培养,最后用血凝血抑对毒株进行分型鉴定。结果 2014年10月-2015年9月间,共采集流感样咽拭子标本759例,核酸阳性标本180例(23.7%),其中季节性H3109例,乙型BY70例,混合型1例(季节性H3和乙型BY混合);细胞培养病毒阳性112株(62.2%)。结论 2014-2015年间天津市第四中心医院所采集的流感样以季节性H3和乙型BY为优势菌株。
Objective To collect influenza-like etiological surveillance from the fourth affiliated hospital of Tianjin No.4 Central Hospital corresponding to the laboratory of Beichen District Center for Disease Control and Prevention from 2014 to 2015 so as to clarify the variation of epidemic strains and strains in 2014-2015. Provide a scientific basis for the development and control of influenza outbreaks. Methods The samples of suspected throat swabs were collected for nucleic acid detection. The positive samples were cultured with MDCK. Finally, the strains were identified by blood coagulation inhibition. Results A total of 759 influenza-like throat swab specimens were collected from October 2014 to September 2015. 180 cases (23.7%) were positive for nucleic acid samples, including seasonal H3109 cases, type B BY70 cases and mixed type cases (season Sexual H3 and B BY mixed); cell culture virus positive 112 (62.2%). Conclusion Influenza samples collected from the Fourth Central Hospital of Tianjin from 2014 to 2015 were predominant strains of seasonal H3 and B-type BY.