论文部分内容阅读
利用结构域交换和密码子优化方法对苏云金芽孢杆菌(Bt)杀虫晶体蛋白Cry1Ab进行合理化设计改造,获得新型Bt蛋白编码基因cry FLIa。利用农杆菌介导法将该基因转入玉米Hi II中,对转基因后代开展分子鉴定和抗性评价等相关工作。结果表明,cryFLIa基因已整合进玉米基因组中,连续3代自交材料检测显示,转基因玉米目标基因正常表达并稳定遗传;蛋白在植株叶片中发育各时期的表达各异,在灌浆期蛋白表达量较高(471.883 ng/g)。室内生测和田间人工接虫试验表明,转cryFLIa基因玉米具有抗亚洲玉米螟的能力。
The Cry1Ab of Bacillus thuringiensis (Bt) insecticidal crystal protein was rationalized by using domain exchange and codon optimization to obtain cry FLIa, a novel Bt protein coding gene. Using Agrobacterium-mediated method, the gene was transferred into Hi-II corn to carry out molecular identification and resistance evaluation of transgenic offspring. The results showed that the cryFLIa gene was integrated into the genome of maize. The three generations of inbred lines showed that the target gene of transgenic maize was expressed normally and stably. The expression of protein in different stages of plant leaves was different. At the grain filling stage, Higher (471.883 ng / g). Indoor bioassay and artificial insects in the field showed that transgenic cryFLIa maize had the ability to resist Asian corn borer.