论文部分内容阅读
目的研究hB7-1基因以重组腺病毒载体转化儿童肝母细胞瘤后,转化细胞的抗肿瘤免疫作用。方法hB7-1基因重组腺病毒载体转入HepG2细胞后,获得高表达B7-1分子的HepG2/hB7-1细胞,MTT法检测HepG2/hB7-1细胞的生长情况,绘制生长曲线;ELISA法检测瘤苗体外对T淋巴细胞IFN-γ表达水平的影响。结果HepG2/hB7-1细胞表面的B7-1分于表达为18.5%,比HepG2细胞表达水平提高16.8倍。从HepG2/hB7-1细胞生长曲线可看到,转化hB7-1后细胞生长速度减慢,生长受到抑制。HepG2/hB7-1瘤苗刺激同种异体T淋巴细胞分泌IFN-γ的量明显超过HepG2细胞(P<0.01)。结论含hB7-1基因的重组腺病毒载体转染HepG2可以获得B7-1分子高表达的细胞株,降低肝母细胞瘤细胞的恶性表征,促进T淋巴细胞分泌IFN-γ,增强抗肿瘤免疫作用。
Objective To study the antitumor immunity of hB7-1 gene transformed into hepatoblastoma cells by recombinant adenovirus vector. Methods HepG2 / hB7-1 cells highly expressing B7-1 were transfected into HepG2 cells with hB7-1 gene. The growth of HepG2 / hB7-1 cells was detected by MTT assay and the growth curve was drawn. The growth curve was detected by ELISA Effect of tumor vaccine on the expression of IFN-γ in T lymphocytes in vitro. Results The expression of B7-1 on HepG2 / hB7-1 cells was 18.5%, which was 16.8 times higher than that of HepG2 cells. From the HepG2 / hB7-1 cell growth curve can be seen, after transforming hB7-1 cell growth slowed down, growth was inhibited. HepG2 / hB7-1 vaccine stimulated allogeneic T lymphocytes to secrete IFN-γ significantly more than HepG2 cells (P <0.01). Conclusion The recombinant adenovirus vector containing hB7-1 gene can be transfected into HepG2 to get the cell line with high expression of B7-1, reduce the malignant characterization of hepatoblastoma cells, promote the secretion of IFN-γ by T lymphocytes and enhance the antitumor immunity .