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目的观察甘草提取物Gc34对MDA-MB-231细胞的体外促凋亡作用。方法 MTT法检测不同时间(24、48、72h),不同浓度(25、50、75、100μg/ml)Gc34对MDA-MB-231细胞增殖的抑制作用;采用AO/EB染色、透射电镜实验判断凋亡;流式细胞仪检测其凋亡率。结果 MTT结果显示,随着Gc34作用浓度升高,细胞增殖能力随之下降,Gc34对MDA-MB-231细胞体外增殖抑制呈明显的剂量-时间依赖性,72h半数抑制浓度(IC50)为65.38μg/ml;AO/EB染色表明药物组细胞出现明显凋亡迹象,而阴性对照组中细胞形态完好;透射电镜结果显示,75μg/ml Gc34处理72h后细胞出现典型凋亡形态学改变;流式细胞仪分析结果显示,随Gc34剂量增加细胞凋亡率逐渐上升,呈明显的剂量依赖性。结论甘草提取物Gc34在体外能明显抑制MDA-MB-231细胞增殖并诱导其凋亡。
Objective To observe the effect of Glycyrrhiza Gc34 on the apoptosis of MDA-MB-231 cells in vitro. Methods MTT assay was used to detect the inhibitory effect of Gc34 on the proliferation of MDA-MB-231 cells at different times (24,48,72h) and different concentrations of Gc34 (25,50,75,100μg / ml). The apoptosis of MDA-MB-231 cells was detected by AO / Apoptosis was detected by flow cytometry. Results The results of MTT assay showed that with the increase of Gc34 concentration, the cell proliferation decreased. Gc34 inhibited the proliferation of MDA-MB-231 cells in a time-and dose-dependent manner. The IC50 of 72 hours was 65.38μg / ml; AO / EB staining showed that the drug group showed obvious signs of apoptosis, while the negative control group, the cell morphology intact; transmission electron microscopy results showed that 75μg / ml Gc34 treated 72h typical apoptotic cells morphological changes; flow cytometry The results of instrument analysis showed that the apoptotic rate increased gradually with dose of Gc34, showing a dose-dependent manner. Conclusion Licorice extract Gc34 can significantly inhibit the proliferation and induce the apoptosis of MDA-MB-231 cells in vitro.