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利用石英晶体微天平(QCM)的高度灵敏性、适配体的特异性捕捉能力和纳米金的信号放大作用,构建了一种QCM生物传感器实现了对Hg~(2+)的检测。将Hg~(2+)适配体自组装于镀金的晶振片表面,作为捕获探针。将Hg~(2+)适配体修饰在纳米金表面,作为链接探针。当Hg~(2+)存在时,捕获探针与链接探针之间形成“T-Hg~(2+)-T”的三明治,使得晶振片上质量增加,从而引起其频率变化。在优化条件下,Hg~(2+)浓度在5~50 nmol/L范围内呈现出良好线性关系,检出限为2 nmol/L(n=10),pb~(2+),As~(3+)等重金属离子无明显干扰。
Based on the high sensitivity of quartz crystal microbalance (QCM), the specific capture ability of aptamers and the signal amplification effect of gold nanoparticles, a QCM biosensor was constructed to detect Hg 2+. Hg ~ (2 +) aptamer self-assembled on the surface of the gold-plated crystal oscillator as a capture probe. Hg ~ (2 +) aptamers were modified on gold nanoparticle surface to serve as linker probes. When Hg 2+ is present, a sandwich of “T-Hg ~ (2 +) - T” is formed between the capture probe and the linker probe, causing an increase in mass on the crystal and causing a change in frequency. Under the optimized conditions, the concentration of Hg 2+ showed a good linearity in the range of 5 ~ 50 nmol / L with the detection limits of 2 nmol / L (n = 10), pb ~ (2 +), As ~ (3 +) and other heavy metal ions without significant interference.