Isolation and identification of compounds from Phaleria macrocarpa (Scheff.) Boerl fruit extract

来源 :Asian Pacific Journal of Tropical Biomedicine | 被引量 : 0次 | 上传用户:zhangxu0202
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Objective: To identify and isolate the chemical compounds of Phaleria macrocarpa(P.macrocarpa) fruit ethanolic extract.Methods: Dried fruit of P.macrocarpa was extracted with 90% ethanol and partitioned between n-hexane/H_2O and ethyl acetate/H_2O.The organic layer was fractionated by various stationary phase and identified by using combined data of 1D [(proton nuclear magnetic resonance(NMR), carbon-13 NMR)], 2D-NMR(heteronuclear multiplequantum correlation and heteronuclear multiple-bond correlation), and mass spectrum.Results: Purification of n-hexane and ethyl acetate fractions from ethanolic extract of P.macrocarpa fruit resulted in isolation of nine compounds.Conclusions: A new compound was isolated and identified as glyceryl pentacosanoate.Also, two xanthones, which are 1,7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone, are firstly reported to be isolated from P.macrocarpa. Objective: To identify and isolate the chemical compounds of Phaleria macrocarpa (P. macrocarpa) fruit ethanolic extract. Methods: Dried fruit of P. macrocarpa was extracted with 90% ethanol and partitioned between n-hexane / H 2 O and ethyl acetate / H 2 O. organic layer was fractionated by various stationary phase and identified by using combined data of 1D [(proton nuclear magnetic resonance (NMR), carbon-13 NMR], 2D-NMR (heteronuclear multiple quantum and correlation and heteronuclear multiple-bond correlation), and mass Results: Purification of n-hexane and ethyl acetate fractions from ethanolic extract of P. macrocarpa fruit resulted in isolation of nine compounds. Conclusions: A new compound was isolated and identified as glyceryl pentacosanoate. Also, two xanthones, which are 1, 7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone, are reported to be isolated from P. macrocarpa.
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