论文部分内容阅读
目的:评价ITS/ITS2热点条形码序列对新疆4种绢蒿属植物及其近缘种银蒿的鉴别能力,为绢蒿属植物分类鉴定提供分子参考依据。方法:对15份绢蒿属及银蒿植物样本进行DNA提取、PCR扩增、双向测序,采用CodonCode Aligner软件对序列峰图进行校对拼接,MEGA6.0分析序列碱基组成及变异,计算K2P遗传距离并构建聚类邻接树,预测ITS2序列二级结构,评估鉴别能力。结果:西北绢蒿、针裂叶绢蒿种内变异位点及信息位点数均小于银蒿。ITS序列种间、种内遗传变异均大于ITS2序列。ITS/ITS2序列邻接(NJ)树均聚为3支,绢蒿属、银蒿1、银蒿2。绢蒿属ITS2序列二级结构与银蒿有差异。结论:通过ITS/ITS2序列的种间K2P遗传距离、邻接树、ITS2序列二级结构分析,ITS、ITS2序列具很好的属鉴定效果,不同产地的针裂叶绢蒿被区分开。
OBJECTIVE: To evaluate the ability of ITS / ITS2 hotspot barcode sequences to identify four species of Seriphidium and their related species, Artemisia selengensis, in Xinjiang, and to provide molecular references for the identification of Seriphidium species. Methods: DNA samples were extracted from 15 samples of Artemisia serrata and Artemisia selengensis. PCR amplification and two-dimensional sequencing were performed. CodonCode Aligner software was used to calibrate the sequence map. MEGA 6.0 was used to analyze the nucleotide composition and variation of the sequence. K2P genetic Distance and construct clustering neighborhood tree to predict secondary structure of ITS2 sequence to evaluate discrimination ability. Results: The species diversity and information loci in Artemisia sphaerocephala and Artemisia sphaerocephala were less than those in Artemisia annua. ITS sequences within the species, within the genetic variation are greater than the ITS2 sequence. ITS / ITS2 sequences were clustered into 3 adjacent trees, Artemisia serrata, Artemisia scabra 1 and Artemisia scabra 2. Silk Artemisia ITS2 sequence secondary structure and there are differences. CONCLUSION: ITS and ITS2 sequences have good genus identification results based on the genetic distance of K2P between ITS / ITS2 sequences and the secondary structure of ITS2 and ITS2 sequences. Serratia aculeatus from different origins is distinguished.