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建立了用反相离子对高效液相色谱法测定人血浆中阿昔洛韦浓度的方法。色谱柱采用ＡｌｌｔｅｃｈＣ＿１８分析柱，以０．０４ｍｏｌ／Ｌ氯化钠－甲醇－ＩＰＲ－Ｂ７离子对色谱试剂混合液（１００：１５：０．６；ｖ／ｖ）为流动相。检测波长为２５４ｎｍ，肾上腺素为内标。血浆样品经高氯酸沉淀蛋白后直接进样测定。阿昔洛韦浓度在４０～１６００ｎｇ／ｍｌ范围内线性良好，ｒ＝０．９９９２．测定含阿苷洛韦１６０ｎｇ／ｍｌ的血浆样品，其日内（ｎ＝７）及日间的ＲＳＤ分别为４．７％和３．０％。平均回收率为９８．２±５．３％。测定了１０名健康志愿者单次口服阿昔洛韦片剂４００ｍｇ后不同时间的血药浓度并计算了有关的药代动力学参数。
A method for the determination of acyclovir in human plasma by reversed-phase ion-pair high performance liquid chromatography was established. AlltechC_18 column was used as the mobile phase, and the mobile phase consisted of 0.04mol / L sodium chloride-methanol-IPR-B7 ion pair chromatographic reagent mixture (100: 15: 0.6; Detection wavelength of 254nm, adrenaline as an internal standard. Plasma samples were precipitated directly by perchloric acid after injection of the sample. Acyclovir concentration in the range of 40 ~ 1600ng / ml good linearity, r = 0.9992. Plasma samples containing 160 ng / ml of acylovir were assayed for intra-day (n = 7) and daytime RSDs of 4.7% and 3.0%, respectively. The average recovery was 98.2 ± 5.3%. The plasma concentrations of 400 mg of acyclovir tablets administered to 10 healthy volunteers at different times were determined and the related pharmacokinetic parameters were calculated.