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正常人尿包括有机物質及无机物質的各种代謝产物,有机物質有尿素、氨基酸、肌酐、尿酸等,无机物质中有氯化物、磷酸盐、硫酸盐等,因其中有些物质可作为細菌生长所必需的营养料,乃試用人尿制作培养基,現将初步获得的结果介紹如下。在实验过程中,一面用人尿培养基,一面用牛肉培养基,进行細菌培养对照。基础培养基: (一) 尿液培养基:取正常人新鮮尿液若干,校正酸醶度至pH7.6—7.8,过滤后分裝,经高压8磅50分钟或15磅20分钟蒸汽灭菌,或用已灭菌的滤菌器过滤以除菌,即可使用。接种大腸杆菌和金黄色葡萄球菌后,在37℃孵箱中培养18—24小时,尿液呈现均匀混浊,长出典型细菌,与用普通肉湯培养基培养出的結果一致。
Normal urine, including organic matter and inorganic substances of various metabolites, organic matter, urea, amino acids, creatinine, uric acid, inorganic substances are chloride, phosphate, sulfate, etc., because some of them can be used as bacteria growth Necessary nutrients, is to try human urine production medium, the preliminary results are presented below. In the course of the experiment, bacterial culture control was performed with human urine medium while using beef medium. Basal medium: (A) urine medium: take a number of normal fresh urine, acidity correction to pH7.6-7.8, filtered and packed, the high pressure 8 pounds for 50 minutes or 15 pounds 20 minutes steam sterilization , Or sterilized bacteria filter to sterilize, you can use. After inoculation of Escherichia coli and Staphylococcus aureus, they were incubated in an incubator at 37 ° C for 18-24 hours. The urine showed uniform turbidity to grow typical bacteria, which was consistent with the results of ordinary broth culture.