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目的研究脂质体镶囊的体内分布及载药后的体外药效。方法运用共沉淀法和层层自组装技术将Cyanine5标记的牛血清白蛋白(Cy5-BSA)包裹在脂质体镶囊的内部,得到荧光标记的脂质体镶囊Cy5-Capsosome;裸鼠尾静脉注射Cy5-Capso-some,利用活体成像系统观察不同时间点其在裸鼠体内的分布情况;采用四甲基偶氮唑盐微量酶反应比色法(MTT)和细胞凋亡实验考查载多柔比星脂质体镶囊对B16F10细胞的细胞毒性和诱导凋亡的能力。结果 Cy5-Capsosome注射后1 h内即到达肺部,并在肺部蓄积,至少滞留12 h,在其他脏器的分布很少;空白脂质体镶囊细胞毒性较低,载多柔比星脂质体镶囊能明显抑制细胞的生长及诱导细胞的凋亡。结论脂质体镶囊具有良好的肺被动靶向性,且具备作为药物载体的潜力。
Objective To study the in vivo distribution of liposomal invagination and drug efficacy in vitro. METHODS Cyanine 5-labeled bovine serum albumin (Cy5-BSA) was encapsulated in liposomes by coprecipitation and layer-by-layer assembly techniques to obtain fluorescent-labeled liposomes Cy5-Capsosome. Intravenous injection of Cy5-Capso-some, the use of live imaging system to observe the distribution of nude mice at different time points; using tetramethyl azoxystrobin trace enzyme reaction (MTT) and apoptosis test to test more Cytotoxicity and the ability of inducing apoptosis of B16F10 cells by ropivacaine liposomes. Results Cy5-Capsosome reached the lungs within 1 h after injection and accumulated in the lungs for at least 12 h, with little distribution in other organs. The cytotoxicity of blank liposomes was low, Liposomal inoculum can significantly inhibit cell growth and induce cell apoptosis. Conclusion The liposomal invagination balloon possesses good lung passive targeting and possesses its potential as a drug carrier.