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目的了解GSM1800MHz射频电磁场对人乳腺癌细胞株MCF7细胞基因表达谱的影响,筛选射频电磁场的可能反应基因,判断射频电磁场对细胞功能的影响。方法体外传代培养的MCF7细胞分为2组,分别接受GSM1800MHz射频电磁场辐照和假辐照24h,辐照组细胞受比吸收率为2.0W/kg或3.5W/kg的射频电磁场间断辐照(5min开、10min关),辐照结束后,立即抽提细胞总RNA,用基因芯片进行全基因转录组水平分析,芯片实验数据采用MAS5.0软件和DMT3.0软件进行分析。采用定量逆转录聚合酶链反应(RTPCR)验证基因芯片分析筛选到的候选差异表达基因。结果MAS软件分析结果表明,MCF7细胞受辐照后,有少量基因的表达水平发生变化。用DMT软件进行重复性和一致性分析后,在比吸收率为2.0W/kg的射频电磁场辐照组,未找到100%一致变化的差异基因;在比吸收率为3.5W/kg的射频电磁场辐照组,找到5个100%一致变化的候选差异基因,但定量RTPCR结果未能验证基因芯片分析筛选到的差异基因。结论在本实验条件下,未能检测到GSM1800MHz射频电磁场明显影响MCF7细胞的基因表达谱,提示所用的射频电磁场辐照不影响本研究中所用的MCF7细胞功能,或该MCF7细胞对射频电磁场辐照反应性低。
Objective To understand the effect of GSM1800MHz radio frequency electromagnetic field on the gene expression profile of human breast cancer cell line MCF7 and to screen the possible gene response to radio frequency electromagnetic field to determine the effect of radio frequency electromagnetic field on cell function. Methods MCF7 cells cultured in vitro were divided into 2 groups: irradiated with GSM1800MHz radio frequency electromagnetic field and sham irradiation respectively for 24 hours, irradiated cells were intermittently irradiated by RF electromagnetic field with specific absorption rate of 2.0W / kg or 3.5W / kg 5min, 10min). After irradiation, the total RNA was extracted and the level of gene transcriptome was analyzed by gene chip. The experimental data were analyzed by MAS5.0 software and DMT3.0 software. Candidate differentially expressed genes selected by microarray analysis using quantitative reverse transcription polymerase chain reaction (RTPCR). Results The results of MAS software showed that the expression of a few genes changed after MCF7 cells were irradiated. Using DMT software for repeatability and consistency analysis, we did not find a 100% consistent difference in the radio-frequency electromagnetic field radiation group with the specific absorption rate of 2.0W / kg; in the RF electromagnetic field with the specific absorption rate of 3.5W / kg Radiation group, five candidate genes with 100% identical changes were found, but the quantitative RT-PCR results failed to validate the differential genes screened by microarray analysis. Conclusion Under the experimental conditions, the lack of detection of GSM1800MHz RF electromagnetic field significantly affects the gene expression profile of MCF7 cells, suggesting that the use of radio frequency electromagnetic field irradiation does not affect the function of MCF7 cells used in this study, or the MCF7 cells irradiated by radio frequency electromagnetic field Reactivity is low.