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目的 从平颏海蛇皮中提取分离糖胺聚糖(GAGs),并对其结构进行初步表征.方法 对海蛇皮进行脱脂处理后,采用木瓜蛋白酶和胰蛋白酶联合酶解提取粗多糖,采用氯代十六烷基吡啶(CPC)沉淀和Q-Sepharose Fast Flow离子交换树脂对粗多糖进行分离纯化.运用醋酸纤维素薄膜电泳分析其GAGs种类,采用1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生高效液相色谱(HPLC)法分析其单糖组成,利用硫酸软骨素酶降解结合质谱和强阴离子交换色谱(SAX-HPLC)法分析其二糖组成.结果 获得了2种GAGs纯化组分,并对其中1种含量较高的纯化组分(HSAP-2)进行了初步结构表征.HSAP-2中含有4种二糖,主要为单硫酸化二糖(4S和6S);单糖组成中含有艾杜糖醛酸(IdoA)、葡萄糖醛酸(GlcA)和乙酰氨基半乳糖(GalNAc);是1种高度杂合的新型硫酸皮肤素(DS).结论 从平颏海蛇皮中分离纯化获得了1种结构新颖的DS,为平颏海蛇多糖结构与生物活性的深入研究提供了基础.“,”Objective To extract and isolate glycosaminoglycans (GAGs) from Lapemis curtus skin,and to preliminarily investigate its structural features.Methods The skin of Lapemis curtus was defatted and digested by papain-trypsase to release glycans.Then the crude polysaccharide was separated by cetylpyridinium chloride (CPC) deposition and Q Sepharose Fast Flow anion-exchange chromatography.The GAGs types of HSAP-2 were determined by cellulose acetate membrane electrophoresis.The monosaccharides composition of HSAP-2 was determined by RP-HPLC with pre-column 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization.The disaccharide composition of HSAP-2 was determined by mass spectrometry and a SAX-HPLC method,after digested by chondroitinase ABC.Results Two GAGs fractions were separated from Lapemis curtus skin,and the structural features of a pure GAGs fraction (HSAP-2) were preliminarily investigated.The disaccharide compositions of HSAP-2 contained four different disaccharides,mainly 4S and 6S.HSAP-2 consisted of iduronic acid(IdoA),glucuronic acid (GlcA) and acetyl galactosamine (GalNAc).It was a new GAGs that mainly contained hybrid dermatan sulfate (DS).Conclusion A novel dermatan sulfate was obtained from Lapemis curtus skin,and our data provided a basis for further research on the relationship of polysaccharide structure and biological activity of Lapemis curtus.