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目的:基于内质网应激通路探讨α-细辛醚诱导人食管癌Eca-109细胞增殖抑制作用及其相关机制。方法:采用MTT法检测α-细辛醚对Eca-109细胞的增殖抑制情况;荧光定量PCR技术检测内质网应激通路相关基因Caspase-4和GRP78 mRNA的表达情况;Western Blot检测Caspase-4和GRP78蛋白表达水平。结果:α-细辛醚可显著抑制Eca-109细胞增殖,并具有一定的剂量时间反应关系;α-细辛醚不同剂量(25μg/ml、50μg/ml)分别作用24h、48h后Caspase-4 mRNA和蛋白的表达减少,GRP78 mRNA和蛋白的表达增加。结论:α-细辛醚对人食管癌Eca-109细胞的增殖具有抑制作用,并呈现明显的剂量时间反应关系,其机制可能是通过上调GRP78,下调Caspase-4的表达实现的。
OBJECTIVE: To investigate the inhibitory effect of α-asarone on the proliferation of human esophageal carcinoma Eca-109 cells and its related mechanisms based on the ER stress pathway. Methods: The inhibitory effect of α-asarone on the proliferation of Eca-109 cells was detected by MTT assay. The expression of caspase-4 and GRP78 mRNA was detected by real-time quantitative PCR. Caspase-4 And GRP78 protein expression levels. Results: α-asarone significantly inhibited the proliferation of Eca-109 cells and had a dose-time response relationship. Caspase-4 was treated with different doses of α-asarone (25μg / ml and 50μg / ml) mRNA and protein expression decreased, GRP78 mRNA and protein expression increased. CONCLUSION: α-asarone can inhibit the proliferation of human esophageal carcinoma cell line Eca-109 and show a dose-response relationship. The mechanism may be through the up-regulation of GRP78 and the down-regulation of Caspase-4 expression.