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目的制备一种新型超声造影剂——纳米液态氟碳脂质微球造影剂,并研究其体外基本特性及聚集后增强超声显影性能。方法采用高压均质法制备纳米液态氟碳脂质微球超声造影剂,检测一般理化性质,再制备生物素化微球,以空白微球为对照组,分别在加入亲和素前后,观察体外聚集情况及超声显影效果,并用DFY超声图像定量分析统计软件计算、比较超声图像平均灰度值。结果所制备的液态氟碳脂质微球为外观呈乳白色的混悬液,镜下微球形态圆整,大小均一,性质稳定,粒径(171.9±91.0)nm;制备的生物素化微球在加入亲和素后聚集成团状,并可增强超声显影,经DFY软件分析,加入亲和素前后超声图像平均灰度值差异有统计学意义。结论成功制备出稳定的纳米液态氟碳脂质超声造影剂,并采用生物素亲和素法证明了该微球具有聚集后增强超声显影性能。
Objective To prepare a new type of ultrasound contrast agent - nanofluidic fluorocarbon lipid microsphere contrast agent and study its basic characteristics in vitro and enhance the ultrasonic imaging performance after aggregation. Methods The nanofluidic fluorocarbon liposomes ultrasound contrast agent was prepared by high pressure homogenization method. The physical and chemical properties were examined. Then the biotinylated microspheres were prepared and the blank microspheres were used as the control group. Before and after the addition of avidin, Aggregation and ultrasonic imaging results, and DFY ultrasound image quantitative analysis of statistical software to calculate, compare the average gray value of ultrasound images. Results The prepared liquid fluorocarbon lipid microspheres were milky white suspension with microscopic morphology, uniform size, stable property and diameter of (171.9 ± 91.0) nm. The prepared biotinylated microspheres After the addition of avidin aggregated into clumps, and can enhance the ultrasound imaging by DFY software analysis, before and after the addition of avidin ultrasound image average gray value difference was statistically significant. Conclusion The stable nano-liquid fluorocarbon lipid ultrasound contrast agent was successfully prepared. The biotin-avidin method was used to prove that the microsphere has the property of enhancing ultrasonic imaging after aggregation.