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目的分析和研究肿瘤坏死因子-α基因(TNF-α)-308与尘肺及尘肺合并结核患者遗传易感性的相关性。方法分别选择103例尘肺患者、51例尘肺合并结核患者以及60例粉尘接触者为研究对象,对其职业史、尘肺病史等进行问卷调查。采用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术检测TNF-α-308位点多态性,最后采用χ2检验对三组研究对象的等位基因频率进行比较,以了解TNF-α-308基因多态性是否与尘肺以及尘肺合并结核易感性相关。结果尘肺组、尘肺合并结核组TNF-α-308等位基因频率与对照组比较,差异无统计学意义(P>0.05)。结论本研究未发现TNF-α-308基因多态性与尘肺、尘肺合并结核发病的遗传易感因素相关。
Objective To analyze and study the relationship between the tumor necrosis factor-α gene (TNF-α) -308 and the genetic susceptibility of patients with pneumoconiosis and pneumoconiosis complicated with tuberculosis. Methods A total of 103 cases of pneumoconiosis, 51 cases of pneumoconiosis with tuberculosis and 60 cases of dust exposure were selected as study subjects, and their occupational history, history of pneumoconiosis were investigated by questionnaire. The polymorphism of TNF-α-308 locus was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Finally, the allele frequencies of three groups of subjects were compared by χ2 test to understand Whether TNF-α-308 gene polymorphism is associated with pneumoconiosis and pneumoconiosis with tuberculosis susceptibility. Results The frequency of TNF-α-308 alleles in pneumoconiosis group and pneumoconiosis combined with tuberculosis group was not significantly different from that in control group (P> 0.05). Conclusion This study found no association between TNF-α-308 gene polymorphism and genetic susceptibility to pneumoconiosis and pneumoconiosis complicated with tuberculosis.