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背景:有研究表明改构型酸性成纤维细胞生长因子是多功能生长因子,但其抗衰老作用至今尚未见报道。目的:观察改构型酸性成纤维细胞生长因子对D-半乳糖致衰老大鼠脑组织、肝组织及血清中超氧化物歧化酶活力、丙二醛含量和抑制羟自由基能力的影响。方法:选择成年Wistar大鼠皮下注射D-半乳糖建立衰老模型,建模成功后随机分为模型组、生理盐水对照组和改构型酸性成纤维细胞生长因子治疗组,另设正常对照组。改构型酸性成纤维细胞生长因子组按12μg/kg剂量肌肉注射改构型酸性成纤维细胞生长因子,生理盐水对照组肌肉注射等量的生理盐水,模型组不作干预。结果与结论:与模型组和生理盐水对照组相比,改构型酸性成纤维细胞生长因子治疗组脑组织、肝组织及血清中超氧化物歧化酶活力和抑制羟自由基能力均显著升高(P<0.01或P<0.05),丙二醛含量均显著降低(P<0.01或P<0.05)。结果证实,改构型酸性成纤维细胞生长因子可通过降低自由基,提高机体的抗氧化能力来发挥延缓衰老的作用。
BACKGROUND: Some studies have shown that the modified acidic fibroblast growth factor is a multi-functional growth factor, but its anti-aging effect has not been reported yet. OBJECTIVE: To observe the effect of modified acidic fibroblast growth factor on superoxide dismutase (SOD), malondialdehyde (MDA) content and hydroxyl radical in brain, serum and liver of aging rats induced by D-galactose. Methods: Aging model was established by subcutaneous injection of D-galactose into adult Wistar rats. The model was randomly divided into model group, normal saline control group and modified acidic fibroblast growth factor treatment group. Another normal control group was established. The modified acidic fibroblast growth factor group was intramuscularly injected with modified acidic fibroblast growth factor at a dosage of 12 μg / kg, and the normal saline control group was injected with the same amount of normal saline intramuscularly, without intervention in the model group. RESULTS AND CONCLUSION: Compared with model group and saline control group, the activity of superoxide dismutase and inhibition of hydroxyl radical in brain tissue, liver tissue and serum were significantly increased in the modified acidic fibroblast growth factor group P <0.01 or P <0.05), MDA content decreased significantly (P <0.01 or P <0.05). The results confirmed that the modified acidic fibroblast growth factor can play a role in anti-aging by reducing free radicals, improve the body’s antioxidant capacity.