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目的:了解新生期小牛视网膜感光细胞联系和细胞连接特征。方法:以新生期小牛视网膜感光细胞为研究对象,扫描电镜和透射电镜为研究手段,实验共用眼球6个,取视网膜24片。结果:新生期小牛视网膜感光细胞胞体平均直径3～5μm。近外核层外侧端,感光细胞间有束状纤维联系;近外核层内侧端,多个感光细胞通过网状纤维形成多种联系,纤维平均直径:0.3～0.6μm。部分感光细胞通过束状纤维呈“会聚式”联系,束状纤维主干平均直径:0.65～0.75μm,分支平均直径:0.25～0.5μm。感光细胞与中间传导细胞建立缝隙连接,部分具备典型结构,缝隙间隙宽度:3～5nm,但大部分感光细胞与中间传导细胞的细胞连接结构尚不成熟。结论:新生期视网膜感光细胞已具有分化和参与功能构建能力,适宜作为视网膜移植供体选材。
OBJECTIVE: To understand neonatal retinal photoreceptor cell connection and cell attachment characteristics. Methods: Neonatal calf retinal photoreceptor cells were used as research objects. Scanning electron microscopy and transmission electron microscopy were used as the research methods. Six eyes were used in the experiment, 24 of which were retina. Results: Neonatal calf retinal photoreceptor cell body average diameter of 3 ~ 5μm. Near the outer nuclear end of the outer layer, there are bundles of fibers between the photoreceptor cells; near the outer end of the nuclear layer, a number of photoreceptor cells through the formation of a variety of network-shaped fibers, average fiber diameter: 0.3 ~ 0.6μm. Part of the photoreceptor cells are in a “convergent” relationship through the bundled fibers. The mean diameter of the bundle fibers is between 0.65 and 0.75 μm and the average diameter of the branches is between 0.25 and 0.5 μm. Photoreceptor cells and intermediate conductive cells to establish a gap junction, part of a typical structure, the gap width of the gap: 3 ~ 5nm, but most of the photoreceptor cells and the middle of the cell conduction structure is not yet mature. Conclusion: Neonatal retinal photoreceptor cells have the ability to differentiate and participate in the function of building, suitable as a retinal donor donor material.